Elkin Kyle R, Riviello John M
Norwegian University of Life Sciences, Institute for Plant and Environmental Science, 1432 Ås, Norway.
Trovion Company, Campbell, CA 95008, United States *Corresponding author. E-mail address:
Talanta. 2014 Feb;119:353-60. doi: 10.1016/j.talanta.2013.11.019. Epub 2013 Nov 20.
Eluent reflux provides a new approach to suppress and reflux (recover) eluent without the continuous generation of chromatographic waste. The current work utilized a device containing ion exchange membranes at the electrodes, in order to prohibit electrolysis gases from entering the eluent stream. Two resin beds (separated by a membrane stack) were responsible for suppressing incoming eluent and regenerating the suppressed eluent to nearly its original concentration after detection. A greater than expected dilution in the eluent concentration was observed as a result of the minor leakage of potassium ions through the anion membrane stack into the electrode chamber. The incomplete recovery of the eluent was offset by the addition of a three port valve (DRV) to regulate eluent concentration. Over 48 h of continuous operation (192 injections), the device's performance was stable (RSD of 0.21% with the three port valve, compared to RSD 3.73% without). The device was able to operate for up to four weeks using 1L of eluent. Chromatograms showing the reproducibility of the device are presented for anions.
洗脱液回流提供了一种抑制和回流（回收）洗脱液的新方法，无需持续产生色谱废物。当前的工作使用了一种在电极处含有离子交换膜的装置，以防止电解气体进入洗脱液流。两个树脂床（由一个膜堆隔开）负责抑制进入的洗脱液，并在检测后将被抑制的洗脱液再生至接近其原始浓度。由于钾离子通过阴离子膜堆少量泄漏到电极室中，观察到洗脱液浓度出现了比预期更大的稀释。通过添加一个三通阀（DRV）来调节洗脱液浓度，抵消了洗脱液回收不完全的问题。在连续运行48小时（192次进样）期间，该装置的性能稳定（使用三通阀时的相对标准偏差为0.21%，相比之下，不使用时为3.73%）。该装置使用1升洗脱液能够运行长达四周。给出了显示该装置对阴离子重现性的色谱图。
