Sun Xiaolu, Tang Jingwen, Chen Weiqing, Zhang Feifang, Yang Bingcheng
Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
J Sep Sci. 2024 Sep;47(18):e202400536. doi: 10.1002/jssc.202400536.
Relative to suppressed conductometric anion exchange chromatography, conductometric ion exclusion chromatography (IEC) is much less popular. Poor limit of detection and manual preparation of eluent are primarily responsible for this. We describe an electrodialytic phosphoric eluent generator for the online production of phosphoric acid eluent at a concentration exceeding 35 mM. It is a sandwiched configuration consisting of three channels isolated by stacked anion exchange membranes and a bipolar membrane plus stacked anion exchange membrane. Such a unique configuration ensures the production of gas-free eluent, obviating the need for a gas removal device. The resulting eluent concentration is controlled by varying the current. Coupled with an ultraviolet absorbance detector, a 0.04-16.67 mg/L limit of detection for model organic acids was achieved by IEC equipped with an electrodialytic phosphoric eluent generator.
与抑制型电导阴离子交换色谱法相比，电导离子排斥色谱法（IEC）的应用要少得多。这主要是由于检测限较差以及洗脱液需手动配制。我们描述了一种用于在线生产浓度超过35 mM的磷酸洗脱液的电渗析磷酸洗脱液发生器。它是一种夹层结构，由通过堆叠的阴离子交换膜和双极膜加堆叠的阴离子交换膜隔离的三个通道组成。这种独特的结构确保了无气体洗脱液的产生，无需气体去除装置。通过改变电流来控制所得洗脱液的浓度。配备电渗析磷酸洗脱液发生器的IEC与紫外吸收检测器联用，对模型有机酸实现了0.04 - 16.67 mg/L的检测限。
