Chun Eun Kyung, Jee Byung Chul, Kim Ju Yeong, Kim Seok Hyun, Moon Shin Yong
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University College of Medicine, Seoul, Korea
Reprod Sci. 2014 Jul;21(7):906-914. doi: 10.1177/1933719113518986. Epub 2014 Jan 8.
The study aimed to investigate the effect of imatinib coadministration on in vitro oocyte acquisition and subsequent embryo development in cyclophosphamide (Cp)-treated mice. Female BDF1 mice were injected with 5 IU equine chorionic gonadotropin (eCG) followed by 5 IU human chorionic gonadotropin 48 hours later and then oocytes were retrieved 14 hours later. Twenty-four hours prior to eCG administration, 25, 50, or 75 mg/kg Cp with or without 7.5 mg/kg imatinib was injected. In the 25 and 50 mg/kg Cp groups, imatinib coadministration significantly enhanced the percentage of mature oocytes (+16.4% and +10.4%) and significantly decreased the percentage of dead oocytes (-25.9% and -15.3%). Imatinib coadministration significantly enhanced the fertilization rate (FR) in the 50 mg/kg Cp group (+12.2%). Intraoocyte spindle integrity was significantly affected by Cp and was rescued by imatinib coadministration. Coadministration of imatinib prior to ovarian stimulation has the benefit of enhancing oocyte maturity and the in vitro FR in Cp-treated mice .
该研究旨在调查伊马替尼联合用药对环磷酰胺（Cp）处理的小鼠体外卵母细胞获取及后续胚胎发育的影响。雌性BDF1小鼠先注射5国际单位马绒毛膜促性腺激素（eCG），48小时后再注射5国际单位人绒毛膜促性腺激素，然后在14小时后采集卵母细胞。在注射eCG前24小时，注射25、50或75毫克/千克的Cp，同时或不同时注射7.5毫克/千克的伊马替尼。在25毫克/千克和50毫克/千克Cp组中，伊马替尼联合用药显著提高了成熟卵母细胞的百分比（分别提高了16.4%和10.4%），并显著降低了死亡卵母细胞的百分比（分别降低了25.9%和15.3%）。伊马替尼联合用药显著提高了50毫克/千克Cp组的受精率（FR）（提高了12.2%）。卵母细胞内纺锤体完整性受到Cp的显著影响，而伊马替尼联合用药可使其恢复。在卵巢刺激前联合使用伊马替尼有利于提高Cp处理小鼠的卵母细胞成熟度和体外受精率。
