参与甘油三酯合成途径的基因多态性以及海洋ω-3多不饱和脂肪酸补充剂可调节血浆甘油三酯水平。

Polymorphisms in genes involved in the triglyceride synthesis pathway and marine omega-3 polyunsaturated fatty acid supplementation modulate plasma triglyceride levels.

作者信息

Ouellette Catherine, Cormier Hubert, Rudkowska Iwona, Guénard Frédéric, Lemieux Simone, Couture Patrick, Vohl Marie-Claude

机构信息

Institute of Nutrition and Functional Foods, Laval University, Quebec, Que., Canada.

出版信息

J Nutrigenet Nutrigenomics. 2013;6(4-5):268-80. doi: 10.1159/000357432. Epub 2014 Jan 3.

DOI:10.1159/000357432

PMID:24401637

Abstract

BACKGROUND

Marine omega-3 (n-3) polyunsaturated fatty acids (PUFA) reduce plasma triglyceride (TG) levels. Genetic factors such as single nucleotide polymorphisms (SNPs) could be responsible for the variability of the plasma TG response to n-3 PUFA supplementation. Previous studies have demonstrated that n-3 PUFA supplementation using fish oil modified the expression levels of three genes involved in the TG synthesis pathway (GPAM, AGPAT3 and AGPAT4) in peripheral blood mononuclear cells.

METHODS

A total of 210 subjects consumed 5 g/day of a fish oil supplement for 6 weeks. Plasma lipids were measured before and after the supplementation period. Three SNPs in GPAM, 13 SNPs in AGPAT3 and 35 SNPs in AGPAT4 were genotyped.

RESULTS

In an ANOVA for repeated measures adjusted for age, sex and BMI, genotype effects on plasma TG levels were observed for rs1838452 in AGPAT3 as well as for rs746731 and rs2293286 in AGPAT4. Genotype × supplementation interaction effects on plasma TG levels were observed for rs2792751 and rs17129561 in GPAM as well as for rs3798943 and rs9458172 in AGPAT4 (p < 0.05).

CONCLUSION

These results suggest that SNPs in genes involved in the TG synthesis pathway may influence plasma TG levels after n-3 PUFA supplementation.

摘要

背景

海洋ω-3（n-3）多不饱和脂肪酸（PUFA）可降低血浆甘油三酯（TG）水平。单核苷酸多态性（SNP）等遗传因素可能是血浆TG对n-3 PUFA补充剂反应变异性的原因。先前的研究表明，使用鱼油补充n-3 PUFA可改变外周血单核细胞中参与TG合成途径的三个基因（GPAM、AGPAT3和AGPAT4）的表达水平。

方法

共有210名受试者每天服用5克鱼油补充剂，持续6周。在补充期前后测量血浆脂质。对GPAM中的3个SNP、AGPAT3中的13个SNP和AGPAT4中的35个SNP进行基因分型。

结果

在针对年龄、性别和BMI进行调整的重复测量方差分析中，观察到AGPAT3中的rs1838452以及AGPAT4中的rs746731和rs2293286对血浆TG水平有基因型效应。观察到GPAM中的rs2792751和rs17129561以及AGPAT4中的rs3798943和rs9458172对血浆TG水平有基因型×补充剂交互作用效应（p<0.05）。