Zendron Carolina, Gonçalves Helder F, Cavalcante Fernanda S, Pereira Thiago R D, Evangelista Alessandra, Ramos Cristiane F, Oliveira Marco Aurelio P
Department of Gynecology, Rio de Janeiro State University, 20551-030, Rio de Janeiro Brazil.
J Ovarian Res. 2014 Jan 8;7:2. doi: 10.1186/1757-2215-7-2.
This study was designed to investigate leptin levels in the fluid in ovarian endometriomas (OEs) and to compare the expression of leptin and its receptors (OBR) in ovarian tissue affected by endometrioma in infertile women to its expression in the normal ovarian tissue of fertile controls without endometriosis.
In this case-control observational study, ovarian tissue, blood samples and peritoneal fluid were obtained from 20 women (10 fertile controls without endometriosis or any ovarian disease, who were undergoing tubal ligation surgery, and 10 infertile women with severe endometriosis and OE). The ovarian endometriomal fluid (EF) was aspirated, and peritoneal-implant (PI) biopsies were performed. The tissues removed during the surgeries were immediately frozen in liquid nitrogen to determine expression levels by western blot and leptin levels by ELISA.
OBR was expressed at higher levels in the ovarian tissue affected by endometrioma than in the normal ovarian tissue (control = 0.38 ± 0.05, study = 0.60 ± 0.09, p = 0.03), but there was no significant difference in leptin levels between these groups (control = 0.57 ± 0.1, study = 0.35 ± 0.1, p = 0.18). Positive and significant correlations were observed between leptin and OBR in the OE (r = 0.85, p = 0.004) and in the PI (r = 0.87, p = 0.001). ELISA results demonstrate a greater leptin concentration within the EF compared with the serum and the PF (serum = 14.25 ± 1.63, PF = 5.98 ± 2.0, EF = 73.8 ± 16.2, p = 0.0001), but there was no correlation between these variables. A positive, significant and strong correlation was observed between PF leptin levels and the expression of leptin and OBR in PI (leptin: r = 0.78, p = 0.007/OBR: r = 0.68, p = 0.04) and between the EF leptin levels and the expression of leptin and OBR in the OE (leptin: r = 0.88, p = 0.008/OBR: r = 0.89, p = 0.005).
These data suggest that leptin may play an important role in the physiopathology of OE through a modulatory interaction with its active receptor.
本研究旨在调查卵巢子宫内膜异位囊肿（OE）液中的瘦素水平，并比较不孕女性受子宫内膜异位症影响的卵巢组织中瘦素及其受体（OBR）的表达与无子宫内膜异位症的正常可育对照卵巢组织中的表达。
在这项病例对照观察性研究中，从20名女性中获取卵巢组织、血液样本和腹腔液（10名无子宫内膜异位症或任何卵巢疾病且正在接受输卵管结扎手术的可育对照，以及10名患有严重子宫内膜异位症和OE的不孕女性）。抽吸卵巢子宫内膜异位囊肿液（EF），并进行腹膜植入物（PI）活检。手术中切除的组织立即在液氮中冷冻，以通过蛋白质印迹法测定表达水平，并通过酶联免疫吸附测定法测定瘦素水平。
受子宫内膜异位症影响的卵巢组织中OBR的表达水平高于正常卵巢组织（对照=0.38±0.05，研究组=0.60±0.09，p=0.03），但这些组之间的瘦素水平无显著差异（对照=0.57±0.1，研究组=0.35±0.1，p=0.18）。在OE（r=0.85，p=0.004）和PI（r=0.87，p=0.001）中观察到瘦素与OBR之间存在正相关且显著相关。酶联免疫吸附测定结果表明，与血清和腹腔液相比，EF中的瘦素浓度更高（血清=14.25±1.63，腹腔液=5.98±2.0，EF=73.8±16.2，p=0.0001），但这些变量之间无相关性。在腹腔液瘦素水平与PI中瘦素和OBR的表达之间（瘦素：r=0.78，p=0.007/OBR：r=0.68，p=0.04）以及EF瘦素水平与OE中瘦素和OBR的表达之间（瘦素：r=0.88，p=0.008/OBR：r=0.89，p=0.005）观察到正相关、显著且强相关。
这些数据表明，瘦素可能通过与其活性受体的调节性相互作用在OE的病理生理学中发挥重要作用。
