Hollender Daiana, Conde Sandra B, Salustio Eduardo, Samartino Luis E
Laboratorio de Brucella, Instituto de Biotecnología, CICVyA, INTA Castelar, Las Cabañas y Nicolás Repetto, Hurlingham (1686), Buenos Aires, Argentina.
AER Lobos - INTA, EEA Pergamino, Centro Regional Buenos Aires Norte, Pergamino, Provincia de Buenos Aires, Argentina.
Rev Argent Microbiol. 2013 Oct-Dec;45(4):229-39. doi: 10.1016/S0325-7541(13)70029-4.
Brucella abortus is the causative agent of bovine brucellosis, a worldwide zoonosis. Up to date, eight biovars of B. abortus have been described. In Argentina, biovar 1 is the most frequently isolated. However, biovar 2, which is more pathogenic than biovar 1, is also found. Molecular methods for subtyping isolates are necessary for allowing epidemiological surveillance and control of eradication programs. Due to the genetic homogeneity of the genus Brucella, the development of molecular typing tools has been difficult. The publication of microorganism genomes facilitates the design of this approach. The aim of this work was to employ a Multiple Locus VNTR Analysis (MLVA) scheme for strains from Argentina isolated in our laboratory. From the 56 isolates analyzed, 47 different genotypic profiles were obtained. All the strains typed as biovar 2 showed the same profile. This scheme allowed assigning each isolate to the biovar it belongs to. All the genotypes were related using the goeBURST analysis and biovar 2 was proposed as founder.
牛布鲁氏菌是牛布鲁氏菌病的病原体，这是一种全球性的人畜共患病。迄今为止，已描述了牛布鲁氏菌的8个生物变种。在阿根廷，生物变种1是最常分离出的类型。然而，也发现了比生物变种1致病性更强的生物变种2。对分离株进行亚型分型的分子方法对于开展流行病学监测和根除计划的控制至关重要。由于布鲁氏菌属的基因同质性，分子分型工具的开发一直很困难。微生物基因组的公布有助于设计这种方法。这项工作的目的是对我们实验室分离的阿根廷菌株采用多位点可变数目串联重复序列分析（MLVA）方案。在分析的56株分离株中，获得了47种不同的基因型谱。所有分型为生物变种2的菌株都显示出相同的谱型。该方案能够将每个分离株归入其所属的生物变种。使用goeBURST分析将所有基因型联系起来，并提出生物变种2为始祖型。
