Bertu Wilson J, Ducrotoy Marie J, Muñoz Pilar M, Mick Virginie, Zúñiga-Ripa Amaia, Bryssinckx Ward, Kwaga Jacob K P, Kabir Junaid, Welburn Susan C, Moriyón Ignacio, Ocholi Reuben A
Brucellosis Research Unit, National Veterinary Research Institute, Vom 930001, Plateau State, Nigeria.
Division of Infection and Pathway, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.
Vet Microbiol. 2015 Oct 22;180(1-2):103-8. doi: 10.1016/j.vetmic.2015.08.014. Epub 2015 Aug 20.
Brucellosis is a worldwide widespread zoonosis caused by bacteria of the genus Brucella. Control of this disease in a given area requires an understanding of the Brucella species circulating in livestock and humans. However, because of the difficulties intrinsic to Brucella isolation and typing, such data are scarce for resource-poor areas. The paucity of bacteriological data and the consequent imperfect epidemiological picture are particularly critical for Sahelian and Sub-Sahara African countries. Here, we report on the characterization of 34 isolates collected between 1976 and 2012 from cattle, sheep and horses in Nigeria. All isolates were identified as Brucella abortus by Bruce-ladder PCR and assigned to biovar 3 by conventional typing. Further analysis by enhanced AMOS-ERY PCR showed that all of them belonged to the 3a sub-biovar, and MLVA analysis grouped them in a cluster clearly distinct from that formed by European B. abortus biovar 3b strains. Nevertheless, MLVA detected heterogeneity within the Nigerian biovar 3a strains. The close genetic profiles of the isolates from cattle, sheep and horses, suggest that, at least in some parts of Nigeria, biovar 3a circulates among animal species that are not the preferential hosts of B. abortus. Consistent with previous genetic analyses of 7 strains from Ivory Cost, Gambia and Togo, the analysis of these 34 Nigerian strains supports the hypothesis that the B. abortus biovar 3a lineage is dominant in West African countries.
布鲁氏菌病是一种由布鲁氏菌属细菌引起的全球广泛传播的人畜共患病。在特定地区控制这种疾病需要了解在牲畜和人类中传播的布鲁氏菌种类。然而，由于布鲁氏菌分离和分型本身存在困难，资源匮乏地区的此类数据很少。细菌学数据的匮乏以及由此导致的不完整的流行病学情况对萨赫勒和撒哈拉以南非洲国家尤为关键。在此，我们报告了1976年至2012年间从尼日利亚的牛、羊和马身上采集的34株分离株的特征。通过布鲁斯梯PCR将所有分离株鉴定为流产布鲁氏菌，并通过传统分型归为生物变种3。增强型AMOS-ERY PCR进一步分析表明，它们均属于3a亚生物变种，多位点可变数目串联重复序列分析（MLVA）将它们归为一个与欧洲流产布鲁氏菌生物变种3b菌株形成的簇明显不同的簇。尽管如此，MLVA检测到尼日利亚生物变种3a菌株内部存在异质性。来自牛、羊和马的分离株的紧密遗传图谱表明，至少在尼日利亚的某些地区，生物变种3a在并非流产布鲁氏菌优先宿主的动物物种之间传播。与之前对来自象牙海岸、冈比亚和多哥的7株菌株的遗传分析一致，对这34株尼日利亚菌株的分析支持了流产布鲁氏菌生物变种3a谱系在西非国家占主导地位的假设。
