Serdjukow S, Kink F, Steigenberger B, Tomás-Gamasa M, Carell T
Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstraße 5-13, 81377 Munich, Germany.
Chem Commun (Camb). 2014 Feb 21;50(15):1861-3. doi: 10.1039/c3cc48937j. Epub 2014 Jan 9.
Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of γ-alkyne modified triphosphate amidates that are converted into a variety of γ-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.
随着化学和技术仪器的改进，实时酶学研究正变得越来越重要。在此，我们报告了γ-炔基修饰的三磷酸酰胺的高效合成，这些化合物通过铜（I）催化的炔烃/叠氮化物点击反应转化为多种γ-荧光团标记的三磷酸酯。合成的三磷酸酯被DNA聚合酶掺入到DNA中。
