Vanas Vanita, Mühlbacher Elsa, Kral Rosana, Sutterlüty-Fall Hedwig
Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, Borschkegasse 8a, 1090, Vienna, Austria.
Tumour Biol. 2014 May;35(5):4447-56. doi: 10.1007/s13277-013-1587-0. Epub 2014 Jan 9.
Sprouty proteins are modulators of mitogen-induced signal transduction processes and therefore can influence the process of cancerogenesis. In particular, Sprouty2 has been shown to have an important role in cancer development of many tumor entities including breast cancer. In this report, we investigated the role of Sprouty4 in breast cancer-derived cell lines. We have found that ectopic Sprouty4 expression inhibits cell proliferation of breast cancer cell lines independently of their endogenous expression levels. Corroborating Sprouty4 downregulation causes accelerated growth. Furthermore, we demonstrate that an increase in Sprouty4 content interferes with serum-induced activation of mitogen-activated protein kinase pathway. Additionally, Sprouty4 expression negatively influences cell migration. These data suggest that Sprouty4 is a possible candidate for a tumor suppressor in breast cancer.
Sprouty蛋白是有丝分裂原诱导的信号转导过程的调节因子，因此能够影响肿瘤发生过程。特别是，Sprouty2已被证明在包括乳腺癌在内的许多肿瘤实体的癌症发展中发挥重要作用。在本报告中，我们研究了Sprouty4在乳腺癌来源细胞系中的作用。我们发现，异位表达Sprouty4可抑制乳腺癌细胞系的细胞增殖，且与它们的内源性表达水平无关。证实Sprouty4下调会导致生长加速。此外，我们证明Sprouty4含量的增加会干扰血清诱导的丝裂原活化蛋白激酶途径的激活。另外，Sprouty4的表达对细胞迁移有负面影响。这些数据表明，Sprouty4可能是乳腺癌中肿瘤抑制因子的一个候选者。
