suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene .

Colorectal cancer (CRC), a common malignant tumor in the digestive tract, is a leading cause of cancer-related death. has been reported to act as a tumor suppressor gene in various tumors. This study aims to assess the role of SPRY4 in colorectal cancer (CRC) and uncover its underlying mechanisms. Firstly, the expression levels of were measured in CRC cell lines. -overexpressing or silencing plasmids were transfected into CRC cells to regulate its expression level. CCK-8, colony formation, EdU assay, wound-healing and Transwell assays were performed to determine cell proliferation, invasion and migration abilities. Then, apoptosis was measured by flow cytometry analysis, and the expression of apoptosis-related protein was analyzed by western-blotting. Next, the tumorigenesis assay was performed in nude mice. According to the results, there was a lower expression of in CRC cell lines compared with normal cell line, and the overexpression of significantly suppressed cell proliferation, migration and invasion, and promoted apoptosis in SW480 cells. Moreover, the enhanced proliferation, invasion and migration upon silencing was reversed by inhibition. In addition, we found that the overexpression of inhibited tumorigenesis by diminishing the size and weight of the tumors. Our study indicates that might be a potential tumor suppressor gene and prognostic factor for patients with CRC.