Department of Gastroenterology, Sunshine Union Hospital, Weifang 261000, China.
School of Life Science, Nanchang University, Nanchang 330031, China.
Aging (Albany NY). 2021 Apr 20;13(8):11665-11677. doi: 10.18632/aging.202859.
Colorectal cancer (CRC), a common malignant tumor in the digestive tract, is a leading cause of cancer-related death. has been reported to act as a tumor suppressor gene in various tumors. This study aims to assess the role of SPRY4 in colorectal cancer (CRC) and uncover its underlying mechanisms. Firstly, the expression levels of were measured in CRC cell lines. -overexpressing or silencing plasmids were transfected into CRC cells to regulate its expression level. CCK-8, colony formation, EdU assay, wound-healing and Transwell assays were performed to determine cell proliferation, invasion and migration abilities. Then, apoptosis was measured by flow cytometry analysis, and the expression of apoptosis-related protein was analyzed by western-blotting. Next, the tumorigenesis assay was performed in nude mice. According to the results, there was a lower expression of in CRC cell lines compared with normal cell line, and the overexpression of significantly suppressed cell proliferation, migration and invasion, and promoted apoptosis in SW480 cells. Moreover, the enhanced proliferation, invasion and migration upon silencing was reversed by inhibition. In addition, we found that the overexpression of inhibited tumorigenesis by diminishing the size and weight of the tumors. Our study indicates that might be a potential tumor suppressor gene and prognostic factor for patients with CRC.
结直肠癌（CRC）是一种常见的消化道恶性肿瘤，是癌症相关死亡的主要原因。已报道 SPRY4 作为一种肿瘤抑制基因在多种肿瘤中发挥作用。本研究旨在评估 SPRY4 在结直肠癌（CRC）中的作用，并揭示其潜在机制。首先，测量了 CRC 细胞系中 SPRY4 的表达水平。将 SPRY4 过表达或沉默质粒转染到 CRC 细胞中，以调节其表达水平。通过 CCK-8、集落形成、EdU 检测、划痕愈合和 Transwell 检测来测定细胞增殖、侵袭和迁移能力。然后，通过流式细胞术分析测定细胞凋亡，通过 Western-blotting 分析测定凋亡相关蛋白的表达。接下来，在裸鼠中进行了 SPRY4 肿瘤发生实验。结果表明，CRC 细胞系中 SPRY4 的表达水平低于正常细胞系，过表达 SPRY4 显著抑制 SW480 细胞的增殖、迁移和侵袭，并促进细胞凋亡。此外，通过抑制 SPRY4 可逆转 SPRY4 沉默引起的增殖、侵袭和迁移增强。此外，我们发现过表达 SPRY4 通过减少肿瘤的大小和重量抑制肿瘤发生。我们的研究表明，SPRY4 可能是 CRC 患者的一种潜在肿瘤抑制基因和预后因素。
