通过靶向新一代测序对耐碳青霉烯类肺炎克雷伯菌分离株进行快速耐药基因组指纹图谱分析和克隆谱系分析。
Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing.
作者信息
Arena Fabio, Rolfe P Alexander, Doran Graeme, Conte Viola, Gruszka Sarah, Clarke Thomas, Adesokan Yemi, Giani Tommaso, Rossolini Gian Maria
机构信息
Department of Medical Biotechnologies, University of Siena, Siena, Italy.
出版信息
J Clin Microbiol. 2014 Mar;52(3):987-90. doi: 10.1128/JCM.03247-13. Epub 2014 Jan 8.
Abstract
Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.
摘要
基于靶向新一代测序（NGS）技术，使用Pathogenica HAI生物检测系统对32株耐碳青霉烯类肺炎克雷伯菌分离株进行了分析，这些分离株代表了不同的耐药机制和克隆谱系。对于大多数菌株，该系统能在更短的时间内，以比目前分子流行病学分析方法更低的劳动强度，同时提供有关相关β-内酰胺耐药决定因素的全面信息，并准确区分克隆谱系。结果支持了基于靶向NGS的技术在类似应用中的实用性。
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