Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Republic of Korea.
Functional Materials Research Group, Korea Food Research Institute, Seongnam, Gyeonggi, Republic of Korea.
Int J Nanomedicine. 2014;9:301-10. doi: 10.2147/IJN.S52414. Epub 2013 Jan 3.
This study determined the effects of oleoresin capsicum (OC) and nanoemulsion OC (NOC) on obesity in obese rats fed a high-fat diet.
THE RATS WERE RANDOMLY SEPARATED INTO THREE GROUPS: a high-fat (HF) diet group, HF + OC diet group, and HF + NOC diet group. All groups were fed the diet and water ad libitum for 14 weeks.
NOC reduced the body weight and adipose tissue mass, whereas OC did not. OC and NOC reduced mRNA levels of adipogenic genes, including peroxisome proliferator-activated receptor (PPAR)-γ, sterol regulatory element-binding protein-1c, and fatty acid-binding protein in white adipose tissue. The mRNA levels of genes related to β-oxidation or thermogenesis including PPAR-α, palmitoyltransferase-1α, and uncoupling protein-2 were increased by the OC and NOC relative to the HF group. Both OC and NOC clearly stimulated AMP-activated protein kinase (AMPK) activity. In particular, PPAR-α, palmitoyltransferase-1α, uncoupling protein-2 expression, and AMPK activity were significantly increased in the NOC group compared to in the OC group. NOC decreased glycerol-3-phosphate dehydrogenase activity whereas OC did not.
From these results, NOC could be suggested as a potential anti-obesity agent in obese rats fed a HF diet. The effects of the NOC on obesity were associated with changes of multiple gene expression, activation of AMPK, and inhibition of glycerol-3-phosphate dehydrogenase in white adipose tissue.
本研究旨在确定辣椒素油树脂(OC)和纳米乳液 OC(NOC)对高脂饮食肥胖大鼠肥胖的影响。
将大鼠随机分为三组:高脂肪(HF)饮食组、HF+OC 饮食组和 HF+NOC 饮食组。所有组均自由进食高脂肪饮食和水 14 周。
NOC 降低了体重和脂肪组织质量,而 OC 则没有。OC 和 NOC 降低了白色脂肪组织中脂肪生成基因的 mRNA 水平,包括过氧化物酶体增殖物激活受体(PPAR)-γ、固醇调节元件结合蛋白-1c 和脂肪酸结合蛋白。与 HF 组相比,OC 和 NOC 增加了与β氧化或产热相关的基因的 mRNA 水平,包括 PPAR-α、棕榈酰转移酶-1α 和解偶联蛋白-2。OC 和 NOC 均明显刺激 AMP 激活蛋白激酶(AMPK)活性。特别是,与 OC 组相比,NOC 组的 PPAR-α、棕榈酰转移酶-1α、解偶联蛋白-2 表达和 AMPK 活性显著增加。NOC 降低了甘油-3-磷酸脱氢酶活性,而 OC 则没有。
从这些结果可以看出,NOC 可作为肥胖大鼠高脂饮食的潜在抗肥胖剂。NOC 对肥胖的影响与白色脂肪组织中多种基因表达的改变、AMPK 的激活以及甘油-3-磷酸脱氢酶的抑制有关。