Kalathia Mitul Babubhai, Shingala Prakash Ashokbhai, Parmar Parin Niranjanbhai, Parikh Yogesh Narenedrabhai, Kalathia Ila Mitulkumar
Department of Paediatrics, Pandit Deendayal Upadhyay Medical College and Hospital, K. T. Sheth Children Hospital, Gujarat, India.
Consultant Microbiologist, Department of Microbiology, Sree Religare Laboratories, Rajkot, Gujarat, India.
J Clin Neonatol. 2013 Oct;2(4):169-72. doi: 10.4103/2249-4847.123092.
Blood culture is gold standard for diagnosis of neonatal sepsis. Low sensitivity of blood culture is usually due to small volume of blood sample, intrapartum antibiotics, and antibiotics given to newborn before sampling.
We evaluated use of Umbilical cord blood culture (UCBC) in diagnosis of neonatal sepsis as compared to peripheral venous blood culture.
This study was done in tertiary care teaching hospital during May-June 2012. A total of 45 newborns with presence of two or more risk factors of sepsis were included.
Blood sample from placental end of umbilical cord was collected and cultured. Primary outcome was diagnosis of neonatal sepsis by use of umbilical cord blood sample as compared with venous blood sample. Secondary outcome was to compare organisms identified by UCBC and venous blood culture.
Sensitivity, specificity, positive and negative predictive values of UCBC were calculated.
A total of 24.44% (11 out of 45) high-risk newborns had positive UCBC. A total of 17.8% (8 out of 45) newborns had positive blood culture report. Organisms grown in UCBC were Pseudomonas (45%, 5 out of 11), Acinetobacter (27.27%, 3 out of 11), Escherichia coli (18.18%, 2 out of 11), and Klebsiella (9%, 1 out of 11).
UCBC is a good method for diagnosis of neonatal sepsis among high-risk newborns as compared to venous blood culture with a sensitivity of 80% and specificity of 91.43%. Organisms grown are comparable to blood culture samples.
血培养是诊断新生儿败血症的金标准。血培养敏感性低通常是由于血样量少、产时使用抗生素以及采样前给新生儿使用抗生素。
我们评估了与外周静脉血培养相比，脐血培养（UCBC）在诊断新生儿败血症中的应用。
本研究于2012年5月至6月在一家三级护理教学医院进行。共纳入45例有两种或更多败血症危险因素的新生儿。
采集脐带胎盘端的血样并进行培养。主要结局是与静脉血样相比，使用脐血样诊断新生儿败血症。次要结局是比较脐血培养和静脉血培养鉴定出的微生物。
计算脐血培养的敏感性、特异性、阳性和阴性预测值。
共有24.44%（45例中的11例）高危新生儿脐血培养呈阳性。共有17.8%（45例中的8例）新生儿血培养报告呈阳性。脐血培养中生长的微生物有铜绿假单胞菌（45%，11例中的5例）、不动杆菌（27.27%，11例中的3例）、大肠杆菌（18.18%，11例中的2例）和克雷伯菌（9%，11例中的1例）。
与静脉血培养相比，脐血培养是诊断高危新生儿败血症的一种好方法，敏感性为80%，特异性为91.43%。培养出的微生物与血培养样本相当。
