Department of General Biology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av, Antônio Carlos, Belo Horizonte 31,270-901, Brazil.
BMC Genomics. 2014 Jan 9;15:14. doi: 10.1186/1471-2164-15-14.
The completion of whole-genome sequencing for Corynebacterium pseudotuberculosis strain 1002 has contributed to major advances in research aimed at understanding the biology of this microorganism. This bacterium causes significant loss to goat and sheep farmers because it is the causal agent of the infectious disease caseous lymphadenitis, which may lead to outcomes ranging from skin injury to animal death. In the current study, we simulated the conditions experienced by the bacteria during host infection. By sequencing transcripts using the SOLiDTM 3 Plus platform, we identified new targets expected to potentiate the survival and replication of the pathogen in adverse environments. These results may also identify possible candidates useful for the development of vaccines, diagnostic kits or therapies aimed at the reduction of losses in agribusiness.
Under the 3 simulated conditions (acid, osmotic and thermal shock stresses), 474 differentially expressed genes exhibiting at least a 2-fold change in expression levels were identified. Important genes to the infection process were induced, such as those involved in virulence, defence against oxidative stress, adhesion and regulation, and many genes encoded hypothetical proteins, indicating that further investigation of the bacterium is necessary. The data will contribute to a better understanding of the biology of C. pseudotuberculosis and to studies investigating strategies to control the disease.
Despite the veterinary importance of C. pseudotuberculosis, the bacterium is poorly characterised; therefore, effective treatments for caseous lymphadenitis have been difficult to establish. Through the use of RNAseq, these results provide a better biological understanding of this bacterium, shed light on the most likely survival mechanisms used by this microorganism in adverse environments and identify candidates that may help reduce or even eradicate the problems caused by this disease.
完成对棒状杆菌 1002 全基因组测序有助于研究该微生物生物学的研究取得重大进展。该细菌会给山羊和绵羊养殖户造成重大损失,因为它是传染性疾病干酪性淋巴结炎的病原体,可能导致从皮肤损伤到动物死亡等各种后果。在本研究中,我们模拟了细菌在宿主感染期间经历的条件。通过使用 SOLiDTM 3 Plus 平台对转录本进行测序,我们鉴定了新的靶点,这些靶点有望增强病原体在不利环境中的生存和复制能力。这些结果还可能确定用于开发疫苗、诊断试剂盒或治疗方法的潜在候选物,以减少农业企业的损失。
在 3 种模拟条件(酸、渗透和热冲击应激)下,鉴定出了 474 个差异表达基因,这些基因的表达水平至少发生了 2 倍的变化。诱导了与感染过程有关的重要基因,如与毒力、抗氧化应激防御、粘附和调节有关的基因,以及许多基因编码假定蛋白,表明需要对细菌进行进一步研究。这些数据将有助于更好地了解棒状杆菌的生物学特性,并有助于研究控制该疾病的策略。
尽管干酪性淋巴结炎的兽医重要性,但该细菌的特征很差;因此,难以建立针对该病的有效治疗方法。通过使用 RNAseq,这些结果提供了对该细菌更好的生物学理解,阐明了该微生物在不利环境中最可能的生存机制,并确定了可能有助于减少甚至消除该疾病带来的问题的候选物。