Department of Biotechnology, Thapar University, Patiala, 147004, Punjab, India.
Appl Biochem Biotechnol. 2014 Mar;172(5):2552-61. doi: 10.1007/s12010-013-0694-0. Epub 2014 Jan 10.
The investigation on the synergistic role of urease (UA) and carbonic anhydrase (CA) in biomineralization of calcium carbonate in Bacillus megaterium suggested that the precipitation of CaCO3 is significantly faster in bacterial culture than in crude enzyme solutions. Calcite precipitation is significantly reduced when both the enzymes are inhibited in comparison with those of the individual enzyme inhibitions indicating that both UA and CA are crucial for efficient mineralization. Carbonic anhydrase plays a role in hydrating carbon dioxide to bicarbonate, while UA aids in maintaining the alkaline pH that promotes calcification process.
研究表明，巨大芽孢杆菌中脲酶（UA）和碳酸酐酶（CA）在碳酸钙生物矿化中的协同作用，细菌培养中的碳酸钙沉淀速度明显快于粗酶溶液。与单独抑制酶相比，当两种酶都被抑制时，方解石沉淀明显减少，这表明 UA 和 CA 对有效的矿化都至关重要。碳酸酐酶在将二氧化碳水合转化为碳酸氢盐方面发挥作用，而 UA 有助于维持促进钙化过程的碱性 pH 值。
