用于米卡芬净治疗药物监测的简单且可靠的液相色谱-串联质谱分析方法。
Simple and robust LC-MS/MS analysis method for therapeutic drug monitoring of micafungin.
作者信息
Boonstra Jasper M, Jongedijk Erwin M, Koster Remco A, Touw Daan J, Alffenaar Jan-Willem C
机构信息
Universityof Groningen, University Medical Center Groningen, Department of ClinicalPharmacy and Pharmacology, Groningen, The Netherlands.
PRA Health Sciences, Research Center, Groningen, The Netherlands.
出版信息
Bioanalysis. 2018 Jun 1;10(11):877-886. doi: 10.4155/bio-2017-0275. Epub 2018 Jun 4.
AIM
To develop a simple and robust LC-MS/MS method to quantify concentrations of micafungin in human plasma for pharmacokinetic studies and therapeutic drug monitoring.
METHODS
Sample preparation involved protein precipitation with acetonitrile:methanol (83:17% v/v) and [C]-micafungin as internal standard. A rapid and selective method for micafungin was validated across a range of 0.200-10.0 mg/l.
RESULTS
The calculated accuracy for the eight-point calibration ranged from 0.7 to 5.3%. Within-run precision ranged from 0.8 to 5.9%, between-run precision ranged from 0.7 to 3.1%, and overall precision ranged from 1.3 to 6.6%.
CONCLUSION
A simple and robust LC-MS/MS method for analyzing micafungin in human plasma has been validated and was utilized for quantification of micafungin.
目的
开发一种简单且可靠的液相色谱-串联质谱法，用于定量测定人血浆中米卡芬净的浓度，以进行药代动力学研究和治疗药物监测。
方法
样品制备包括用乙腈：甲醇（83:17% v/v）和[C] - 米卡芬净作为内标进行蛋白沉淀。在0.200 - 10.0 mg/l范围内验证了一种快速且选择性的米卡芬净测定方法。
结果
八点校准的计算准确度范围为0.7%至5.3%。批内精密度范围为0.8%至5.9%，批间精密度范围为0.7%至3.1%，总体精密度范围为1.3%至6.6%。
结论
一种用于分析人血浆中米卡芬净的简单且可靠的液相色谱-串联质谱法已得到验证，并用于米卡芬净的定量测定。
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