Nadeem Afzal, Javaid Khursheed, Sami Waqas, Zafar Abu, Jahan Shah, Zaman Shakeela, Nagi Ah
Department of lmmunology, University of Health Sciences, Lahore, Pakistan.
Departments of Biostatics, University of Health Sciences, Lahore, Pakistan.
Clin Lab. 2013;59(11-12):1311-7. doi: 10.7754/clin.lab.2013.121140.
Diabetes mellitus (DM) is a health concern because it leads to complications such as retinopathy. Pakistan has 6.9 million DM affected people that will double by 2025. A study was designed to determine the level of IL-17 in the serum of Pakistani type 2 diabetes mellitus (T2DM) patients.
It was a cross-sectional case-control study that included 212 subjects. Subjects without diabetes were labeled as Group-I (30 healthy volunteers), Group-II (30 T2DM without retinopathy), and Group-III (152 T2DM with retinopathy). The serum level of IL-17 was determined by ELISA technique. Data was analysed using SPSS 17.0 and one way ANOVA to observe group mean differences.
More females were in Group-II (83%) and Group-III (66%) compared to Group-I (30%). The age of subjects was higher in Group-III (50 years) and Group-II (49 years) compared to Group-I (34 years). Group-III had longer mean duration of disease (10.51 years) than Group-II (7.76 years). Group-I had increased levels of IL-17 followed by Group-II and Group-III. On comparison, statistically significant differences were observed among the three groups, and between Group-I and Group-III, but there was no significant difference between Group-I and Group-II, nor between Group-II and Group-III. Further, on comparison of age, gender, and duration of disease there were significant differences while there was no significant difference between the percentages of HbA1c.
Age, gender, and duration of diabetes may contribute in the development of T2DM retinopathy while serum level of IL-17 was inversely associated with T2DM and retinopathy.
糖尿病（DM）是一个健康问题，因为它会导致诸如视网膜病变等并发症。巴基斯坦有690万糖尿病患者，到2025年这一数字将翻倍。一项研究旨在测定巴基斯坦2型糖尿病（T2DM）患者血清中白细胞介素-17（IL-17）的水平。
这是一项横断面病例对照研究，包括212名受试者。无糖尿病的受试者被标记为第一组（30名健康志愿者）、第二组（30名无视网膜病变的T2DM患者）和第三组（152名有视网膜病变的T2DM患者）。采用酶联免疫吸附测定（ELISA）技术测定血清IL-17水平。使用SPSS 17.0软件和单因素方差分析对数据进行分析，以观察组间均值差异。
与第一组（30%）相比，第二组（83%）和第三组（66%）中的女性更多。与第一组（34岁）相比，第三组（50岁）和第二组（49岁）受试者的年龄更大。第三组疾病的平均病程（10.51年）比第二组（7.76年）更长。第一组IL-17水平升高，其次是第二组和第三组。经比较，三组之间以及第一组和第三组之间观察到统计学上的显著差异，但第一组和第二组之间、第二组和第三组之间没有显著差异。此外，在年龄、性别和病程的比较上存在显著差异，而糖化血红蛋白（HbA1c）百分比之间没有显著差异。
年龄、性别和糖尿病病程可能在T2DM视网膜病变的发生中起作用，而血清IL-17水平与T2DM和视网膜病变呈负相关。
