血清白细胞介素-6和白细胞介素-17与2型糖尿病视网膜病变呈负相关。

Negative association of serum IL-6 and IL-17 with type-II diabetes retinopathy.

作者信息

Afzal Nadeem, Zaman Shakeela, Asghar Aneela, Javed Khursheed, Shahzad Faheem, Zafar Abu, Nagi A H

机构信息

Department of Immunology, University of Health Sciences, Lahore, Pakistan, e-mail:

出版信息

Iran J Immunol. 2014 Mar;11(1):40-8.

PMID:24632587

Abstract

BACKGROUND

Diabetes mellitus (DM) is a health concern which leads to complications such as retinopathy. Pakistan has 6.9 million people living with DM and this toll will be doubled by 2025.

OBJECTIVE

To determine serum IL-6 and IL-17 of type 2 diabetes mellitus (T2DM) patients with retinopathy.

METHODS

In this cross-sectional case-control study, 212 subjects enrolled which were categorized into 3 groups. Group-I included 30 subjects without diabetes, group-II consisted of 30 subjects with T2DM without retinopathy and group-III consisted of 152 subjects with T2DM and retinopathy. Serum IL-6 and IL-17 levels were determined by ELISA. Data was analysed using SPSS 17.0 and one way ANOVA to observe group mean differences.

RESULTS

Longer mean duration of disease was detected in group-III than group-II (p=0.007). Highest IL-6 level was detected in group-II and highest IL-17 level was detected in group-I. For IL-6, significant differences were detected among groups in total, between Group-I and Group-III and between Group-II and Group-III (p<0.0001 each). Regarding IL-17, significant differences were found among groups in total (p=0.002) and between Group-I and Group-III (p=0.001). No significant difference in the percentages of HbA1c observed between groups.

CONCLUSIONS

Age, gender and duration of diabetes contribute to T2DM retinopathy. Serum IL-6 and IL-17 were inversely associated with T2DM retinopathy.

摘要

背景

糖尿病是一个备受关注的健康问题，可导致视网膜病变等并发症。巴基斯坦有690万人患有糖尿病，到2025年这一数字将翻倍。

目的

测定患有视网膜病变的2型糖尿病（T2DM）患者的血清白细胞介素-6（IL-6）和白细胞介素-17（IL-17）水平。

方法

在这项横断面病例对照研究中，纳入了212名受试者，分为3组。第一组包括30名无糖尿病的受试者，第二组由30名患有T2DM但无视网膜病变的受试者组成，第三组由152名患有T2DM且有视网膜病变的受试者组成。采用酶联免疫吸附测定法（ELISA）测定血清IL-6和IL-17水平。使用SPSS 17.0软件和单因素方差分析对数据进行分析，以观察组间均值差异。

结果

第三组的疾病平均病程长于第二组（p = 0.007）。第二组检测到的IL-6水平最高，第一组检测到的IL-17水平最高。对于IL-6，各组之间、第一组和第三组之间以及第二组和第三组之间均检测到显著差异（每组p < 0.0001）。关于IL-17，各组之间总体存在显著差异（p = 0.002），第一组和第三组之间也存在显著差异（p = 0.001）。各组之间糖化血红蛋白（HbA1c）百分比无显著差异。