Wang Hua-Cheng, Chen Cha, Wang Li-Na, Long Yi-Fei, Zhang Wei-Zheng, Li You-Qiang, Xiao Qian, Yuan Hui
Clinical Laboratory Department, Guangzhou Brain Hospital, Guangzhou Medical University, China.
Clinical Laboratory Department, Guangdong Provincial Hospital of Traditional Chinese Medicine, China.
Clin Lab. 2013;59(11-12):1429-32. doi: 10.7754/clin.lab.2013.130213.
The rapid increase of syphilis underscores a tremendous need to carefully evaluate many new serological tests for syphilis and choose efficient and economical strategies for syphilis screening, especially in the case of primary infection with low antibody titer. Between 2011 and 2012, 73 patients' sera samples were included in this retrospective study. They were either TRUST or TPPA reactive, either LA (latex agglutination) based auto3 TP or CLIA (chemiluminescence assay) based Architect Syphilis TP assay reactive. The contradictory weak response samples were further examined by FTA-Abs method. TPPA could not give reactive results in samples with antibody concentration less than 10 mIU. Auto3 TP reagent shows good linearity at low antibody titers and was more sensitive than TPPA, while the former does not show significant superiority compared to the Architect Syphilis TP assay at low antibody titer, except that it is suitable for adaptation on diverse automated chemistry analyzers.
梅毒发病率的快速上升凸显了对众多新型梅毒血清学检测方法进行仔细评估并选择高效且经济的梅毒筛查策略的迫切需求，尤其是在原发性感染且抗体滴度较低的情况下。2011年至2012年期间，本回顾性研究纳入了73例患者的血清样本。这些样本要么TRUST或TPPA呈阳性反应，要么基于乳胶凝集试验（LA）的Auto3 TP或基于化学发光免疫分析（CLIA）的Architect梅毒螺旋体（TP）检测呈阳性反应。对反应结果矛盾的弱阳性样本进一步采用荧光密螺旋体抗体吸收试验（FTA - Abs）方法进行检测。TPPA在抗体浓度低于10 mIU的样本中无法得出阳性结果。Auto3 TP试剂在低抗体滴度时显示出良好的线性，且比TPPA更敏感，不过在低抗体滴度时，与Architect梅毒螺旋体TP检测相比，前者未显示出显著优势，只是它适用于多种自动化化学分析仪。
