Fluorescent Lab, Division of Medicinal and Process Chemistry, ‡Electron Microscopy Unit, §Division of Pharmaceutics, and ∥Division of Toxicology, CSIR-Central Drug Research Institute (CDRI) , Lucknow 226031, India.
Org Lett. 2014 Feb 7;16(3):756-9. doi: 10.1021/ol403470d. Epub 2014 Jan 10.
A new class of live cell permeant, nontoxic fluoranthene-based fluorescent probe (FLUN-550) having a high Stokes shift in aqueous medium has been discovered. It showed selective staining of lipid droplets (LDs, dynamic cytoplasmic organelles) at a low concentration without background noise in in vitro live cell imaging of 3T3-L1 preadipocytes, J774 macrophages, MCF7 breast cancer cells, and single-celled, parasitic protozoa Leishmania donovani promastigotes and in vivo nonparasitic soil nematode C. elegans.
一种新型的活细胞通透、非毒性的荧蒽基荧光探针（FLUN-550）已被发现，它在水相中有较大的斯托克斯位移。在体外 3T3-L1 前脂肪细胞、J774 巨噬细胞、MCF7 乳腺癌细胞和单细胞寄生原生动物利什曼原虫前鞭毛体的活细胞成像中，该探针在低浓度下显示出对脂滴（LD，细胞质动态细胞器）的选择性染色，而没有背景噪声，在体内非寄生土壤线虫 C. elegans 中也有同样的效果。
