Wu Andrew, Kolanowski Jacek L, Boumelhem Badwi B, Yang Kylie, Lee Rebecca, Kaur Amandeep, Fraser Stuart T, New Elizabeth J, Rendina Louis M
School of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia.
Discipline of Physiology, School of Medical Sciences, The University of Sydney, Sydney, NSW, 2006, Australia.
Chem Asian J. 2017 Jul 18;12(14):1704-1708. doi: 10.1002/asia.201700423. Epub 2017 Jun 22.
The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane-containing coumarin, 5, which is sufficiently lipophilic to localise in cellular lipid droplets. In non-polar solvents which show comparable polarities to those of a lipid environment, compound 5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging. Compound 5 can stain lipid droplets in ex vivo adipocytes as well as in cultured cells, and can be utilised in flow cytometry as well as confocal microscopy.
荧光标记物和探针的使用极大地促进了生物学研究，但这依赖于提供一系列具有不同性质的荧光团。在此，我们报告了一种新型的含碳硼烷香豆素（化合物5），它具有足够的亲脂性，能够定位于细胞脂滴中。在与脂质环境极性相当的非极性溶剂中，化合物5的荧光量子产率比在水性溶剂中高出两个数量级，这为脂滴成像增加了进一步的选择性。化合物5能够对离体脂肪细胞以及培养细胞中的脂滴进行染色，并且可用于流式细胞术以及共聚焦显微镜检查。
