Sharma Ashutosh, Umar Shahida, Kar Parmita, Singh Kavita, Sachdev Monika, Goel Atul
Fluorescent Lab, Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
Analyst. 2016 Jan 7;141(1):137-43. doi: 10.1039/c5an01623a. Epub 2015 Nov 3.
We discovered a new class of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts. The salient features of the probe are its visible excitation maximum, aqueous compatibility, selectivity and remarkable stability (for more than a week) in live cells, even better than commercially available Nile red.
我们发现了一类新型无毒、高荧光且具有生物相容性的探针AFN，可用于在固定和活的人宫颈癌细胞（HeLa）以及3T3-L1前脂肪细胞中对细胞内脂滴（LDs）进行选择性染色，且无任何背景伪影。该探针的显著特点包括其可见激发最大值、水相兼容性、选择性以及在活细胞中具有出色的稳定性（超过一周），甚至优于市售的尼罗红。
