一种用于细胞内脂滴固定和活细胞成像的新型生物相容性荧光探针AFN。

A new type of biocompatible fluorescent probe AFN for fixed and live cell imaging of intracellular lipid droplets.

作者信息

Sharma Ashutosh, Umar Shahida, Kar Parmita, Singh Kavita, Sachdev Monika, Goel Atul

机构信息

Fluorescent Lab, Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.

出版信息

Analyst. 2016 Jan 7;141(1):137-43. doi: 10.1039/c5an01623a. Epub 2015 Nov 3.

DOI:10.1039/c5an01623a

PMID:26528832

Abstract

We discovered a new class of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts. The salient features of the probe are its visible excitation maximum, aqueous compatibility, selectivity and remarkable stability (for more than a week) in live cells, even better than commercially available Nile red.

摘要

我们发现了一类新型无毒、高荧光且具有生物相容性的探针AFN，可用于在固定和活的人宫颈癌细胞（HeLa）以及3T3-L1前脂肪细胞中对细胞内脂滴（LDs）进行选择性染色，且无任何背景伪影。该探针的显著特点包括其可见激发最大值、水相兼容性、选择性以及在活细胞中具有出色的稳定性（超过一周），甚至优于市售的尼罗红。