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World J Microbiol Biotechnol. 1995 May;11(3):280-3. doi: 10.1007/BF00367098.
Shigella dysenteriae type 1, isolated locally, was found to contain six plasmids and these could be eliminated using SDS. A 70-kb plasmid was necessary to maintain the normal cell-wall morphology. Synthesis of nine major membrane proteins (90 to 40 kDa) was severely impaired in all-plasmid cured strains. Electron microscopy revealed a prominent separation between the outer and inner membranes of the cured strains, indicating that plasmid loss led to defects in the cell envelope. The growth rates of the strains having only the 70-kb plasmid and the plasmidless strain were 3- to 30-fold less than in the wild type strain.
当地分离的志贺氏痢疾杆菌 1 型含有 6 种质粒，用 SDS 可以消除这些质粒。一个 70kb 的质粒是维持正常细胞壁形态所必需的。所有消除质粒的菌株中，9 种主要膜蛋白（90 到 40kDa）的合成受到严重损害。电子显微镜显示，消除质粒的菌株的外膜和内膜之间明显分离，表明质粒丢失导致细胞包膜缺陷。只有 70kb 质粒和无质粒菌株的生长速度比野生型菌株慢 3 到 30 倍。
