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基于生物反应器的植物组织与细胞培养进展:挑战与前景

Bioreactor-based advances in plant tissue and cell culture: challenges and prospects.

作者信息

Valdiani Alireza, Hansen Ole Kim, Nielsen Ulrik Braüner, Johannsen Vivian Kvist, Shariat Maryam, Georgiev Milen I, Omidvar Vahid, Ebrahimi Mortaza, Tavakoli Dinanai Elham, Abiri Rambod

机构信息

a Department of Geosciences and Natural Resource Management, Section for Forest, Nature and Biomass, Faculty of Science , University of Copenhagen , Frederiksberg C 1958 , Denmark.

b Department of Food Science, Faculty of Food Science and Technology , Universiti Putra Malaysia , Serdang , Selangor 43400 UPM , Malaysia.

出版信息

Crit Rev Biotechnol. 2018 Nov 15;39(1):20-34. doi: 10.1080/07388551.2018.1489778.

DOI:10.1080/07388551.2018.1489778
PMID:30431379
Abstract

Bioreactors are engineered systems capable of supporting a biologically active situation for conducting aerobic or anaerobic biochemical processes. Stability, operational ease, improved nutrient uptake capacity, time- and cost-effectiveness, and large quantities of biomass production, make bioreactors suitable alternatives to conventional plant tissue and cell culture (PTCC) methods. Bioreactors are employed in a wide range of plant research, and have evolved over time. Such technological progress, has led to remarkable achievements in the field of PTCC. Since the classification of bioreactors has been extensively reviewed in numerous reviews, the current article avoids repeating the same material. Alternatively, it aims to highlight the principal advances in the bioreactor hardware s used in PTCC rather than classical categorization. Furthermore, our review summarizes the most significant steps as well as current state-of-the-art of PTCC carried out in various types of bioreactor.

摘要

生物反应器是经过工程设计的系统,能够支持进行需氧或厌氧生化过程的生物活性环境。稳定性、操作简便性、提高的养分吸收能力、时间和成本效益以及大量生物质生产,使生物反应器成为传统植物组织和细胞培养(PTCC)方法的合适替代方案。生物反应器被广泛应用于各种植物研究中,并随着时间的推移不断发展。这种技术进步在PTCC领域取得了显著成就。由于生物反应器的分类在众多综述中已得到广泛探讨,本文避免重复相同内容。相反,它旨在突出用于PTCC的生物反应器硬件的主要进展,而非传统分类。此外,我们的综述总结了在各种类型生物反应器中进行的PTCC的最重要步骤以及当前的技术水平。

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