Lehrstuhl für Zellbiologie, Institut für Biologie II, Schänzlestraße 1, D-7800, Freiburg, Federal Republic of Germany.
Planta. 1978 Jan;141(3):303-9. doi: 10.1007/BF00388348.
Isolation of tubulous chromoplasts from Tropaeolum majus L. petals was achieved in pure form. Their main substructures-lipid bodies, tubules, and envelope membranes-have been enriched. Whole chromoplasts as well as substructures have been tested for their activities in lipid synthesis. The following activities were found: fatty acid synthesis from acetate, glycosyl transfer reactions from UDP-galactose and UDP-glucose to galactolipids and sterols, acyltransferase reactions from palmitoyl-CoA, and a very active acyl-CoA hydrolase (EC 3.1.2.2.). Fatty acid synthesis was restricted to whole chromoplasts. Glycosyl- and acyltransferases were essentially confined to envelope membranes, whereas acyl-CoA hydrolase was found in all fractions. The chemical composition of chromoplast subfractions was determined. The lipid bodies consisted mainly of galactolipids and carotenoid esters in a 1:1 ratio, together with small amounts of protein.
从金莲花花瓣中分离出管状质体，得到纯质体。它们的主要亚结构——脂体、小管和包膜膜——得到了富集。整个质体和亚结构都被检测到具有脂质合成的活性。发现了以下活性：从乙酸合成脂肪酸，从 UDP-半乳糖和 UDP-葡萄糖向半乳糖脂和甾醇转移糖基转移反应，从棕榈酰-CoA 进行酰基转移反应，以及非常活跃的酰基辅酶 A 水解酶（EC 3.1.2.2.）。脂肪酸合成仅限于整个质体。糖基转移酶和酰基转移酶主要局限于包膜膜，而酰基辅酶 A 水解酶存在于所有级分中。测定了质体亚级分的化学成分。脂体主要由半乳糖脂和类胡萝卜素酯以 1:1 的比例组成，还含有少量蛋白质。
