Van Hummel H C, Hulsebos T H, Wintermans J F
Biochim Biophys Acta. 1975 Feb 20;380(2):219-26. doi: 10.1016/0005-2760(75)90008-9.
When either mitochondria, chloroplast stroma lamellae, or osmotically shocked chloroplasts were centrifuged through sucrose gradients, zones were always obtained at the 0.6 M-0.9 M boundary which were highly active in galactosyltransferase. These activities did not coincide with maxima for chlorophyll or cytochrome c oxidase activity. A second chlorophyll-free fraction was obtained at lower density, showing high galactosyltransferase activity when incubated after isolation. The results indicate that the highly active fractions originate from chloroplast envelopes.
当线粒体、叶绿体基质片层或经渗透压冲击的叶绿体通过蔗糖梯度离心时，总能在0.6M - 0.9M边界处获得对半乳糖基转移酶高度活跃的区带。这些活性与叶绿素或细胞色素c氧化酶活性的最大值不一致。在较低密度处获得了第二个无叶绿素的组分，分离后孵育时显示出高半乳糖基转移酶活性。结果表明，这些高活性组分源自叶绿体被膜。
