Kleinig H, Liedvogel B
Eur J Biochem. 1978 Feb;83(2):499-505. doi: 10.1111/j.1432-1033.1978.tb12116.x.
Isolated daffodil (Narcissus pseudonarcissus) chromoplasts showed high rates of [14C]acetate incorporation into lipids. The fatty acids synthesized were predominantly palmitic acid (93%). The radioactivity incorporated was shared mainly between long-chain acyl-CoA (25%), free fatty acids (24%), phosphatidic acid (17%), diacylglycerol (15%), and phosphatidycholine (11%). Galactolipids were not labelled. ATP, NaHCO3, and also the structural integrity of the organelles were essential. Omission of exogenous CoA led to a decreased incorporation (49%); under these conditions the label was distributed mainly between free fatty acids (66%) and diacylglycerol (19%). Addition of lysophosphatidylcholine increased the labelling of phosphatidylcholine, whereas addition of glycerol 1-phosphate increased the labelling of phosphatidic acid and diacylglycerol. Acyl-CoA synthetase and acyl thioesterase (acyl-Coa) activities could be demonstrated. The results are discussed in terms of chromoplasts as non-photosynthetic organelles exhibiting high lipid-synthesizing capabilities.
分离出的水仙（黄水仙）质体显示出较高的[14C]乙酸掺入脂质的速率。合成的脂肪酸主要是棕榈酸（93%）。掺入的放射性主要分布在长链酰基辅酶A（25%）、游离脂肪酸（24%）、磷脂酸（17%）、二酰基甘油（15%）和磷脂酰胆碱（11%）之间。半乳糖脂未被标记。ATP、NaHCO3以及细胞器的结构完整性都是必不可少的。省略外源辅酶A会导致掺入减少（49%）；在这些条件下，标记主要分布在游离脂肪酸（66%）和二酰基甘油（19%）之间。添加溶血磷脂酰胆碱会增加磷脂酰胆碱的标记，而添加1-磷酸甘油会增加磷脂酸和二酰基甘油的标记。可以证明存在酰基辅酶A合成酶和酰基硫酯酶（酰基辅酶A）活性。本文从质体作为具有高脂质合成能力的非光合细胞器的角度对结果进行了讨论。
