Laboratory of Microbial Ecology, Faculty of Agricultural and Applied Biological Sciences, University of Gent, Coupure Links 653, B-9000, Gent, Belgium.
World J Microbiol Biotechnol. 1995 Sep;11(5):529-35. doi: 10.1007/BF00286368.
Two protocols for following soil methane enrichment were used, one with methane dosed as a carbon source ([C]-soil) and one with methane plus minerals ([C+M]-soil). Methane oxidation occurred much faster in soil receiving minerals in addition to methane than in the control soil receiving only methane. In both treatments, only a small fraction of methane (2% to 14%) was converted into microbial biomass C. Nevertheless, a strong increase in soil microbial biomass (up to 1.5 to 2.0-fold) was achieved in the [C+M]-soil in a 3-week period. Due to methane application, the NO3 (-) content of the soil was significantly decreased, by 83% to 90% in the [C]-soil and by 56% to 83% in the [C+M]-soil. Soil enzymatic activities were slightly increased in the [C+M]-soil only. The soil-methane incubation did not alter the composition of the monitored microbial populations in the soil or in rhizosphere of plants. In the [C]-soil, methane incubation resulted in reduction of the shoot dry wt of maize by 8% to 12%. In the [C+M]-soil under non-limiting mineral-nutrient status, a significant increase in shoot dry wt was observed for maize (13%), a neutral effect was registered for spinach and a negative effect was observed for wheat.
采用了两种跟踪土壤甲烷富集的方案，一种是将甲烷作为碳源添加（[C]-土壤），另一种是将甲烷与矿物质一起添加（[C+M]-土壤）。与仅接收甲烷的对照土壤相比，接收甲烷和矿物质的土壤中的甲烷氧化速度要快得多。在这两种处理中，只有一小部分甲烷（2%至 14%）转化为微生物生物量 C。然而，在 3 周的时间内，[C+M]-土壤中的土壤微生物生物量（高达 1.5 到 2.0 倍）显著增加。由于甲烷的应用，土壤中的硝酸盐（NO3（-））含量显著降低，[C]-土壤中的含量降低了 83%至 90%，[C+M]-土壤中的含量降低了 56%至 83%。仅在[C+M]-土壤中，土壤酶活性略有增加。土壤甲烷培养并未改变土壤或植物根际中监测到的微生物种群的组成。在[C]-土壤中，甲烷培养导致玉米地上部分干重减少了 8%至 12%。在非限制矿物质营养状态下的[C+M]-土壤中，玉米地上部分干重显著增加（13%），菠菜的效果为中性，而小麦的效果为负。
