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World J Microbiol Biotechnol. 1995 Nov;11(6):631-7. doi: 10.1007/BF00361005.
A laboratory upflow anaerobic sludge blanket reactor, seeded with fine, suspended, bacterial floc with 1.76 g volatile suspended solids/l, was used to treat synthetic methanolic waste. After 180 days of continuous peration, granular sludge with discrete granules of 1 to 2 mm diam. was formed, with 52 g volatile suspended solids/l. Granules were brown, relatively soft and had a settling velocity of 1.61 cm/s. Extracellular polymeric matter extracted from the granular sludge had high carbohydrate content but low nucleic acid content. The ash of the granular sludge contained Na(+), K(+) and Mg(2+) up to 15.0, 11.7 and 3.75 mg/g, respectively. Scanning and transmission electron microscopy revealed that the granular sludge was dominated by methanogens resembling Methanosarcina.
采用接种细小悬浮絮状细菌（污泥浓度 1.76gVSS/L）的上流式厌氧污泥床反应器处理甲醇合成废水，经过 180 天连续运行，形成了粒径为 1~2mm 的颗粒污泥，污泥浓度达到 52gVSS/L。颗粒污泥呈褐色，比较松软，沉降速度为 1.61cm/s。从颗粒污泥中提取的胞外聚合物含有较高的碳水化合物，但核酸含量较低。颗粒污泥的灰分中含有高达 15.0、11.7 和 3.75mg/g 的 Na(+)、K(+)和 Mg(2+)。扫描电子显微镜和透射电子显微镜显示，颗粒污泥主要由类似于 Methanosarcina 的产甲烷菌组成。
