Gilbert E J
Department of Biochemistry, University of Leicester, UK.
Enzyme Microb Technol. 1993 Aug;15(8):634-45. doi: 10.1016/0141-0229(93)90062-7.
Lipases (glycerol ester hydrolases; EC 3.1.1.3) are important enzymes which, due to their ability to catalyze a number of reactions, are receiving considerable interest from both academia and industry. The bacterial genus Pseudomonas is a prolific producer of a number of extracellular enzymes including lipase. This review summarizes the biochemical properties and recent advances in the molecular genetic analysis of a wide variety of Pseudomonas lipases. In particular, a comparison is made between the amino acid sequences of the various lipases as well as their secondary gene products, which are thought to be essential for secretion of the enzyme.
脂肪酶（甘油酯水解酶；EC 3.1.1.3）是重要的酶，由于它们能够催化多种反应，正受到学术界和工业界的广泛关注。假单胞菌属是包括脂肪酶在内的多种胞外酶的丰富生产者。这篇综述总结了多种假单胞菌脂肪酶的生化特性以及分子遗传分析的最新进展。特别地，对各种脂肪酶的氨基酸序列及其二级基因产物进行了比较，这些被认为对酶的分泌至关重要。
