碳源对嗜热真菌灰绿曲霉β-葡萄糖苷酶系统的影响。
Effect of carbon source on the β-glucosidase system of the thermophilic fungus Humicola grisea.
机构信息
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出版信息
World J Microbiol Biotechnol. 1996 May;12(3):297-9. doi: 10.1007/BF00360933.
H. grisea produced an extracellular β-glucosidase (EC 3.2.1.21) at high activity in media supplemented with carboxymethyl cellulose (CMC) or cellobiose. Cellobiose-induced β-glucosidase was insensitive to glucose repression whereas that of CMC-supplemented cultures was partially repressed. Molecular sieving revealed three main active components (Mr 50, 128 and 240 kDa). Glucose competitively inhibited β-glucosidase activities with Ki values of 0.9MM and 3.3MM (extracellular) and 10.2MM and 22.6MM (cytosolic), induced in the presence of CMC or cellobiose respectively.
灰色犁头霉在含有羧甲基纤维素（CMC）或纤维二糖的培养基中能够产生高活性的胞外β-葡萄糖苷酶（EC 3.2.1.21）。纤维二糖诱导的β-葡萄糖苷酶对葡萄糖抑制不敏感，而 CMC 补充培养物中的酶则部分受到抑制。分子筛显示有三个主要的活性成分（Mr 50、128 和 240 kDa）。葡萄糖与胞外和胞内的β-葡萄糖苷酶活性均具有竞争性抑制作用，Ki 值分别为 0.9MM 和 3.3MM（在 CMC 存在的情况下）和 10.2MM 和 22.6MM（在纤维二糖存在的情况下）。
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