肉豆蔻酸对“醛”突变体中细菌生物发光的刺激作用。
Myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants.
作者信息
Ulitzur S, Hastings J W
出版信息
Proc Natl Acad Sci U S A. 1978 Jan;75(1):266-9. doi: 10.1073/pnas.75.1.266.
Abstract
The involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark "aldehyde" mutants. We have found that the luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. This stimulation has been demonstrated in aldehyde mutants of two species of luminous bacteria, Beneckea harveyi and Photobacterium fischeri. The responses, both in intensity and yield, are proportional to the amount of added tetradecanoic acid over a 1000-fold range, down to 10 pmol ml-1. Unsaturated long chain fatty acids are potent inhibitors of the tetradecanoic acid stimulation, but they do not effect the in vivo luminescence of wild-type bacteria.
摘要
长链醛参与细菌发光，这一点已为人所知，一方面是因为在与纯荧光素酶的体外反应中发光需要它，另一方面是因为它能够在体内刺激某一类暗“醛”突变体发光。我们发现，某些（但并非所有）此类醛突变体的发光也受到长链脂肪族脂肪酸的刺激，其中十四烷酸（肉豆蔻酸）具有显著的特异性。这种刺激作用已在两种发光细菌——哈维氏贝内克氏菌和费氏发光杆菌的醛突变体中得到证实。在强度和产量方面，响应与添加的十四烷酸量在1000倍的范围内成正比，低至10皮摩尔/毫升。不饱和长链脂肪酸是十四烷酸刺激作用的有效抑制剂，但它们对野生型细菌的体内发光没有影响。
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本文引用的文献
1
BIOCHEMICAL CHARACTERISTICS OF ALDEHYDE AND LUCIFERASE MUTANTS OF LUMINOUS BACTERIA.发光细菌醛和荧光素酶突变体的生化特性
Proc Natl Acad Sci U S A. 1955 Feb 15;41(2):67-70. doi: 10.1073/pnas.41.2.67.
2
THE INFLUENCE OF ALDEHYDE CHAIN LENGTH UPON THE RELATIVE QUANTUM YIELD OF THE BIOLUMINESCENT REACTION OF ACHROMOBACTER FISCHERI.
J Biol Chem. 1963 Sep;238:3100-5.
3
Enzymic determination of aldehyde permeability in luminous bacteria. I. Effect of chain length on light emission and penetration.发光细菌中醛渗透性的酶法测定。I. 链长对发光和渗透的影响。
Arch Biochem Biophys. 1958 May;75(1):87-105. doi: 10.1016/0003-9861(58)90400-4.
4
Isolation, identification, and function of long chain fatty aldehydes affecting the bacterial luciferin-luciferase reaction.
J Biol Chem. 1954 Nov;211(1):213-25.
5
Factors affecting the luminescence of cell-free extracts of the luminous bacterium, Achromobacter fischeri.影响费氏无色杆菌发光菌无细胞提取物发光的因素。
Arch Biochem Biophys. 1953 Nov;47(1):16-33. doi: 10.1016/0003-9861(53)90434-2.
6
A stable, inexpensive, solid-state photomultiplier photometer.一种稳定、廉价的固态光电倍增管光度计。
Anal Biochem. 1971 Jan;39(1):243-50. doi: 10.1016/0003-2697(71)90481-7.
7
Mutant analysis and enzyme subunit complementation in bacterial bioluminescence in Photobacterium fischeri.费氏弧菌细菌生物发光中的突变体分析和酶亚基互补
J Bacteriol. 1970 Oct;104(1):300-12. doi: 10.1128/jb.104.1.300-312.1970.
8
Cellular control of the synthesis and activity of the bacterial luminescent system.细菌发光系统合成与活性的细胞控制
J Bacteriol. 1970 Oct;104(1):313-22. doi: 10.1128/jb.104.1.313-322.1970.
9
Structurally distinct bacterial luciferases.结构不同的细菌荧光素酶。
Biochemistry. 1969 Dec;8(12):4681-9. doi: 10.1021/bi00840a004.
10
Temperature-sensitive mutants of bioluminescent bacteria.发光细菌的温度敏感突变体。
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