Karimi Ehsan, Mehrabanjoubani Pooyan, Keshavarzian Maryam, Oskoueian Ehsan, Jaafar Hawa Z E, Abdolzadeh Ahmad
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400, UPM Serdang, Selangor, Malaysia.
J Sci Food Agric. 2014 Aug;94(11):2324-30. doi: 10.1002/jsfa.6567. Epub 2014 Feb 26.
Plant foods are rich sources of bioactive compounds that can act as antioxidants to prevent heart disease, reduce inflammation, reduce the incidence of cancers and diabetes. This study aimed to determine the phenolics and flavonoids profiling in three varieties of rice straw and five varieties of the seed husk of Iranian rice using high-performance liquid chromatography (HPLC). Furthermore, the antioxidant activities of the extracts were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and nitric oxide assays.
HPLC analyses showed that the gallic acid, pyrogallol, apigenin and rutin were the main phenolic and flavonoid compounds in all varieties of rice. In addition, the methanolic extracts of Hashemi and Ali Kazemi varieties showed the highest amounts of phenolic and flavonoid contents, respectively. Rice straw and husk of Iranian varieties showed considerable antioxidant activity and Hashemi indicated significantly (P < 0.01) higher DPPH and nitric oxide free radical scavenging activities when compared to the other varieties.
The present study revealed that rice straw and seed husk of Iranian varieties shows high antioxidant activities and they contain various types of phenolic and flavonoid compounds that could be use in food and medical industries.
植物性食物是生物活性化合物的丰富来源,这些化合物可作为抗氧化剂预防心脏病、减轻炎症、降低癌症和糖尿病的发病率。本研究旨在利用高效液相色谱法(HPLC)测定伊朗水稻三个品种的稻草和五个品种的稻壳中的酚类和黄酮类化合物谱。此外,通过2,2-二苯基-1-苦基肼(DPPH)自由基和一氧化氮测定法评估提取物的抗氧化活性。
HPLC分析表明,没食子酸、连苯三酚、芹菜素和芦丁是所有水稻品种中的主要酚类和黄酮类化合物。此外,哈希米品种和阿里·卡泽米品种的甲醇提取物分别显示出最高的酚类和黄酮类含量。伊朗品种的稻草和稻壳表现出相当高的抗氧化活性,与其他品种相比,哈希米品种的DPPH和一氧化氮自由基清除活性显著更高(P < 0.01)。
本研究表明,伊朗品种的稻草和稻壳具有较高的抗氧化活性,并且含有多种酚类和黄酮类化合物,可用于食品和医药行业。