Piperidis Nathalie
SRA Limited, Mackay, QLD, Australia.
Methods Mol Biol. 2014;1115:325-36. doi: 10.1007/978-1-62703-767-9_16.
Genomic in situ hybridization (GISH) is an invaluable cytogenetic technique which enables the visualization of whole genomes in hybrids and polyploidy taxa. Total genomic DNA from one or two different species/genome is used as a probe, labeled with a fluorochrome and directly detected on mitotic chromosomes from root-tip meristems. In sugarcane we were able to characterize interspecific hybrids of two closely related species as well as intergeneric hybrids of two closely related genera.
基因组原位杂交（GISH）是一种非常有价值的细胞遗传学技术，它能够使杂种和多倍体分类群中的整个基因组可视化。来自一个或两个不同物种/基因组的总基因组DNA用作探针，用荧光染料标记，并直接在根尖分生组织的有丝分裂染色体上进行检测。在甘蔗中，我们能够鉴定两个近缘物种的种间杂种以及两个近缘属的属间杂种。
