Characterization of chromosome composition of sugarcane in nobilization by using genomic in situ hybridization.

BACKGROUND

Interspecific hybridization is an effective strategy for germplasm innovation in sugarcane. Nobilization refers to the breeding theory of development and utilization of wild germplasm. is the main donor of resistance and adaptive genes in the nobilization breeding process. Chromosome transfer in sugarcane is complicated; thus, research of different inheritance patterns can provide guidance for optimal sugarcane breeding.

RESULTS

Through chromosome counting and genomic in situ hybridization, we found that six clones with 80 chromosomes were typical and four other clones with more than 80 chromosomes were interspecific hybrids between and . These data support the classical view that is characterized by 2n = 80. In addition, genomic in situ hybridization showed that five F clones were products of a 2n + n transmission and one F clone was the product of an n + n transmission in clear pedigree noble hybrids between and . Interestingly, Yacheng 75-408 and Yacheng 75-409 were the sibling lines of the F progeny from the same parents but with different genetic transmissions.

CONCLUSIONS

This is the first clear evidence of Loethers, Crystallina, Luohanzhe, Vietnam Niuzhe, and Nanjian Guozhe were typical by GISH. Furthermore, for the first time, we identified the chromosome transmission of six F hybrids between and . These findings may provide a theoretical basis for germplasm innovation in sugarcane breeding and guidance for further sugarcane nobilization.