Yu Fan, Wang Ping, Li Xueting, Huang Yongji, Wang Qinnan, Luo Ling, Jing Yanfen, Liu Xinlong, Deng Zuhu, Wu Jiayun, Yang Yongqing, Chen Rukai, Zhang Muqing, Xu Liangnian
1National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
2Guangdong Provincial Bioengineering Institute, Guangzhou Sugarcane Industry Research Institute, Guangzhou, China.
Mol Cytogenet. 2018 Jun 7;11:35. doi: 10.1186/s13039-018-0387-z. eCollection 2018.
Interspecific hybridization is an effective strategy for germplasm innovation in sugarcane. Nobilization refers to the breeding theory of development and utilization of wild germplasm. is the main donor of resistance and adaptive genes in the nobilization breeding process. Chromosome transfer in sugarcane is complicated; thus, research of different inheritance patterns can provide guidance for optimal sugarcane breeding.
Through chromosome counting and genomic in situ hybridization, we found that six clones with 80 chromosomes were typical and four other clones with more than 80 chromosomes were interspecific hybrids between and . These data support the classical view that is characterized by 2n = 80. In addition, genomic in situ hybridization showed that five F clones were products of a 2n + n transmission and one F clone was the product of an n + n transmission in clear pedigree noble hybrids between and . Interestingly, Yacheng 75-408 and Yacheng 75-409 were the sibling lines of the F progeny from the same parents but with different genetic transmissions.
This is the first clear evidence of Loethers, Crystallina, Luohanzhe, Vietnam Niuzhe, and Nanjian Guozhe were typical by GISH. Furthermore, for the first time, we identified the chromosome transmission of six F hybrids between and . These findings may provide a theoretical basis for germplasm innovation in sugarcane breeding and guidance for further sugarcane nobilization.
种间杂交是甘蔗种质创新的有效策略。高贵化是指野生种质开发利用的育种理论。在高贵化育种过程中是抗性和适应性基因的主要供体。甘蔗中的染色体转移很复杂；因此，对不同遗传模式的研究可为优化甘蔗育种提供指导。
通过染色体计数和基因组原位杂交，我们发现6个具有80条染色体的克隆是典型的，另外4个具有80条以上染色体的克隆是与之间的种间杂种。这些数据支持了经典观点，即的特征是2n = 80。此外，基因组原位杂交表明，在与之间清晰谱系的高贵杂种中，5个F克隆是2n + n传递的产物，1个F克隆是n + n传递的产物。有趣的是，崖城75 - 408和崖城75 - 409是来自同一亲本的F后代的同胞系，但具有不同的遗传传递方式。
这是通过基因组原位杂交首次明确证明Loethers、Crystallina、罗汉蔗、越南牛蔗和南涧果蔗是典型的。此外，我们首次确定了与之间6个F杂种的染色体传递情况。这些发现可能为甘蔗育种中的种质创新提供理论基础，并为进一步的甘蔗高贵化提供指导。
