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利用基因组原位杂交技术揭示桑属植物的染色体组成和遗传关系。

Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization.

机构信息

State Key Laboratory of Resource Insects, Southwest University, Beibei, Chongqing, 400715, China.

出版信息

BMC Plant Biol. 2023 Sep 15;23(1):428. doi: 10.1186/s12870-023-04448-9.

DOI:10.1186/s12870-023-04448-9
PMID:37710184
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10503058/
Abstract

BACKGROUND

Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events.

RESULTS

Comparative genomic in situ hybridization (cGISH) and self-GISH were performed to illustrate the chromosome constitution and genetic relationships of 40 mulberry accessions belonging to 12 species and three varietas in the Morus genus and containing eight different ploidy levels. We identified six homozygous cGISH signal patterns and one heterozygous cGISH signal pattern using four genomic DNA probes. Using cGISH and self-GISH data, we defined five mulberry sections (Notabilis, Nigra, Wittiorum, and Cathayana, all contained only one species; and Alba, which contained seven closely related species and three varietas, was further divided into two subsections) and proposed the genetic relationships among them. Differential cGISH signal patterns detected in section Alba allowed us to refine the genetic relationships among the closely related members of this section.

CONCLUSIONS

We propose that GISH is an efficient tool to investigate the chromosome constitution and genetic relationships in mulberry. The results obtained here can be used to guide outbreeding of heterozygous perennial crops like mulberry.

摘要

背景

桑树（Morus spp.）是一种经济上重要的木本植物，已经有几千年的养蚕（丝绸养殖）历史。由于多倍体和频繁的杂交事件，桑树的遗传背景较为复杂。

结果

本研究通过比较基因组原位杂交（cGISH）和自 GISH 实验，阐明了来自 12 个种和 3 个变种的 40 个桑树种质资源的染色体组成和遗传关系，这些种质资源包含 8 个不同的倍性水平。我们使用 4 个基因组 DNA 探针鉴定了 6 种纯合 cGISH 信号模式和 1 种杂合 cGISH 信号模式。利用 cGISH 和自 GISH 数据，我们定义了 5 个桑树种（Notabilis、Nigra、Wittiorum 和 Cathayana，均只包含 1 个种；Alba 包含 7 个近缘种和 3 个变种，进一步细分为 2 个亚组），并提出了它们之间的遗传关系。在 Alba 种中检测到的差异 cGISH 信号模式使我们能够细化该种内近缘成员之间的遗传关系。

结论

我们提出 GISH 是研究桑树染色体组成和遗传关系的有效工具。这里的结果可用于指导异花授粉，例如杂种多年生作物桑树的选育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/8598ac25b1bd/12870_2023_4448_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/e3f47f7d6c9e/12870_2023_4448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/9732c7480761/12870_2023_4448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/f96bf5b113dc/12870_2023_4448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/00f8db571ac9/12870_2023_4448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/6ee4e411444c/12870_2023_4448_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/79e0bc936120/12870_2023_4448_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/aeb620f9eea2/12870_2023_4448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/5b27ec10f904/12870_2023_4448_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/8598ac25b1bd/12870_2023_4448_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/e3f47f7d6c9e/12870_2023_4448_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/9732c7480761/12870_2023_4448_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/f96bf5b113dc/12870_2023_4448_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/00f8db571ac9/12870_2023_4448_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/6ee4e411444c/12870_2023_4448_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/79e0bc936120/12870_2023_4448_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/aeb620f9eea2/12870_2023_4448_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/5b27ec10f904/12870_2023_4448_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f39/10503058/8598ac25b1bd/12870_2023_4448_Fig9_HTML.jpg

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