Wang Boyi, Tan Hua-Wei, Fang Wanping, Meinhardt Lyndel W, Mischke Sue, Matsumoto Tracie, Zhang Dapeng
Yunnan Forestry Technological College , Kunming 650224, Yunnan, China ; Sustainable Perennial Crops Laboratory, USDA-ARS, Beltsville Agricultural Research Center , Beltsville, MD 20705, USA.
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, Jiangsu, China.
Hortic Res. 2015 Jan 14;2:14065. doi: 10.1038/hortres.2014.65. eCollection 2015.
Longan (Dimocarpus longan Lour.) is an important tropical fruit tree crop. Accurate varietal identification is essential for germplasm management and breeding. Using longan transcriptome sequences from public databases, we developed single nucleotide polymorphism (SNP) markers; validated 60 SNPs in 50 longan germplasm accessions, including cultivated varieties and wild germplasm; and designated 25 SNP markers that unambiguously identified all tested longan varieties with high statistical rigor (P<0.0001). Multiple trees from the same clone were verified and off-type trees were identified. Diversity analysis revealed genetic relationships among analyzed accessions. Cultivated varieties differed significantly from wild populations (F st=0.300; P<0.001), demonstrating untapped genetic diversity for germplasm conservation and utilization. Within cultivated varieties, apparent differences between varieties from China and those from Thailand and Hawaii indicated geographic patterns of genetic differentiation. These SNP markers provide a powerful tool to manage longan genetic resources and breeding, with accurate and efficient genotype identification.
龙眼（Dimocarpus longan Lour.）是一种重要的热带果树作物。准确的品种鉴定对于种质管理和育种至关重要。利用公共数据库中的龙眼转录组序列，我们开发了单核苷酸多态性（SNP）标记；在50份龙眼种质资源（包括栽培品种和野生种质）中验证了60个SNP；并指定了25个SNP标记，这些标记以高统计严谨性（P<0.0001）明确鉴定了所有测试的龙眼品种。对来自同一克隆的多棵树进行了验证，并鉴定出变异树。多样性分析揭示了所分析种质资源之间的遗传关系。栽培品种与野生群体存在显著差异（F st=0.300；P<0.001），表明在种质保存和利用方面存在未开发的遗传多样性。在栽培品种中，中国品种与泰国和夏威夷品种之间存在明显差异，表明存在遗传分化的地理模式。这些SNP标记为管理龙眼遗传资源和育种提供了一个强大的工具，能够进行准确高效的基因型鉴定。
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