Shah Kausar Hussain, Almaghrabi Bachar, Bohlmann Holger
Plant Mol Biol Report. 2013;31(6):1529-1538. doi: 10.1007/s11105-013-0614-z.
Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose. Each of them is reported to be highly efficient, robust and cost-effective. Here, a variety of expression vectors which were designed for transient and stable plant transformation, including pPZP3425, pPZP5025, pPZPTRBO, pJLTRBO, pEAQ- and pBY030-2R, was compared for the expression of green fluorescent protein and β-glucuronidase in by -mediated transient expression. Our results show that pPZPTRBO, pJLTRBO and pEAQ- had comparable expression levels without co-infiltration of a RNA-silencing inhibitor. The other vectors, including the non-viral vectors pPZP5025 and pPZP3425, needed co-infiltration of the RNA-silencing inhibitor P19 to give good expression levels.
植物中重组蛋白的生产在实际应用中愈发重要。然而，稳定转化的转基因植物的生产是一个漫长的过程。另一方面，瞬时表达可以在一周内产生重组蛋白，并且为此构建了许多病毒载体。据报道，它们中的每一个都具有高效、稳健且具有成本效益的特点。在此，比较了多种为植物瞬时和稳定转化设计的表达载体，包括pPZP3425、pPZP5025、pPZPTRBO、pJLTRBO、pEAQ-和pBY030-2R，用于通过农杆菌介导的瞬时表达来表达绿色荧光蛋白和β-葡萄糖醛酸酶。我们的结果表明，在不共浸润RNA沉默抑制剂的情况下，pPZPTRBO、pJLTRBO和pEAQ-具有相当的表达水平。其他载体，包括非病毒载体pPZP5025和pPZP3425，需要共浸润RNA沉默抑制剂P19才能获得良好的表达水平。
