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与植物相关的共生伯克霍尔德氏菌缺乏哺乳动物致病所需的标志性策略。

Plant-associated symbiotic Burkholderia species lack hallmark strategies required in mammalian pathogenesis.

作者信息

Angus Annette A, Agapakis Christina M, Fong Stephanie, Yerrapragada Shailaja, Estrada-de los Santos Paulina, Yang Paul, Song Nannie, Kano Stephanie, Caballero-Mellado Jésus, de Faria Sergio M, Dakora Felix D, Weinstock George, Hirsch Ann M

机构信息

Dept. of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, California, United States of America.

Baylor College of Medicine, Houston, Texas, United States of America.

出版信息

PLoS One. 2014 Jan 8;9(1):e83779. doi: 10.1371/journal.pone.0083779. eCollection 2014.

Abstract

Burkholderia is a diverse and dynamic genus, containing pathogenic species as well as species that form complex interactions with plants. Pathogenic strains, such as B. pseudomallei and B. mallei, can cause serious disease in mammals, while other Burkholderia strains are opportunistic pathogens, infecting humans or animals with a compromised immune system. Although some of the opportunistic Burkholderia pathogens are known to promote plant growth and even fix nitrogen, the risk of infection to infants, the elderly, and people who are immunocompromised has not only resulted in a restriction on their use, but has also limited the application of non-pathogenic, symbiotic species, several of which nodulate legume roots or have positive effects on plant growth. However, recent phylogenetic analyses have demonstrated that Burkholderia species separate into distinct lineages, suggesting the possibility for safe use of certain symbiotic species in agricultural contexts. A number of environmental strains that promote plant growth or degrade xenobiotics are also included in the symbiotic lineage. Many of these species have the potential to enhance agriculture in areas where fertilizers are not readily available and may serve in the future as inocula for crops growing in soils impacted by climate change. Here we address the pathogenic potential of several of the symbiotic Burkholderia strains using bioinformatics and functional tests. A series of infection experiments using Caenorhabditis elegans and HeLa cells, as well as genomic characterization of pathogenic loci, show that the risk of opportunistic infection by symbiotic strains such as B. tuberum is extremely low.

摘要

伯克霍尔德氏菌属是一个多样且动态变化的属,包含致病物种以及与植物形成复杂相互作用的物种。致病菌株,如类鼻疽伯克霍尔德氏菌和鼻疽伯克霍尔德氏菌,可在哺乳动物中引发严重疾病,而其他伯克霍尔德氏菌菌株则是机会致病菌,会感染免疫系统受损的人类或动物。尽管已知一些机会致病性伯克霍尔德氏菌病原体可促进植物生长甚至固氮,但对婴儿、老年人和免疫功能低下人群的感染风险不仅导致其使用受限,还限制了非致病性共生物种的应用,其中一些共生物种可使豆科植物根部长瘤或对植物生长产生积极影响。然而,最近的系统发育分析表明,伯克霍尔德氏菌物种可分为不同的谱系,这表明在农业环境中安全使用某些共生物种具有可能性。共生谱系中还包括许多促进植物生长或降解外源化合物的环境菌株。这些物种中的许多都有潜力在肥料不易获取的地区促进农业发展,并可能在未来作为接种剂用于在受气候变化影响的土壤中生长的作物。在这里,我们使用生物信息学和功能测试来研究几种共生伯克霍尔德氏菌菌株的致病潜力。一系列使用秀丽隐杆线虫和人宫颈癌细胞系的感染实验,以及对致病基因座的基因组特征分析表明,诸如块茎伯克霍尔德氏菌等共生菌株引发机会性感染的风险极低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cd5/3885511/ca693b406090/pone.0083779.g001.jpg

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