Key Laboratory of Animal Virology of Ministry of Agriculture, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
College of Animal Sciences and Technology, Zhejiang A&F University, Lin'an, Zhejiang, People's Republic of China.
PLoS One. 2014 Jan 9;9(1):e84503. doi: 10.1371/journal.pone.0084503. eCollection 2014.
Infectious bursal disease virus (IBDV) infection causes immunodeficiency in chickens. To understand cell-mediated immunity during IBDV infection, this study perform a detailed analysis of chicken γc chain (chCD132) and γc family cytokines, including interleukins 2, 4, 7, 9, and 15. The mouse anti-chCD132 monoclonal antibody (mAb) was first generated by the E.coli-expressed γc protein. Immunofluorescence assay further showed that γc was a protein located with the anti-chCD132 mAb on the surface of chicken's splenic mononuclear cells. Real-time quantitative RT-PCR revealed that the chCD132 mRNA transcript was persistently downregulated in embryo fibroblasts, spleen and thymus of chickens infected with IBDV. Correspondingly during IBDV infection, the transcription of five γc family cytokines was downregulated in the thymus and presented an imbalance in the spleen. Fluorescence-activated cell sorting analyses also indicated that the percentage of CD132(+)CD8(+) T cells linearly decreased in the bursa of IBDV-infected chickens. These results confirmed that IBDV infection disturbed the in vivo balance of CD132 and γc family cytokine expression and that IBDV-induced immunodeficiency involved cellular networks related to the γc family.
传染性腔上囊病病毒（IBDV）感染会导致鸡的免疫功能缺陷。为了了解 IBDV 感染期间的细胞介导免疫反应，本研究对鸡 γc 链（chCD132）和 γc 家族细胞因子进行了详细分析，包括白细胞介素 2、4、7、9 和 15。首先通过大肠杆菌表达的 γc 蛋白生成了鼠抗 chCD132 单克隆抗体（mAb）。免疫荧光分析进一步表明，γc 是一种位于鸡脾单核细胞表面的与抗 chCD132 mAb 结合的蛋白。实时定量 RT-PCR 显示，IBDV 感染的鸡胚成纤维细胞、脾脏和胸腺中 chCD132 mRNA 转录物持续下调。相应地，在 IBDV 感染期间，胸腺和脾脏中五种 γc 家族细胞因子的转录呈下调趋势，呈现出不平衡状态。荧光激活细胞分选分析还表明，IBDV 感染鸡的腔上囊中 CD132(+)CD8(+)T 细胞的比例呈线性下降。这些结果证实，IBDV 感染扰乱了体内 CD132 和 γc 家族细胞因子表达的平衡，IBDV 诱导的免疫缺陷涉及与 γc 家族相关的细胞网络。
