三七总皂苷对肝纤维化大鼠细胞因子的影响

[Effect of Panax notoginseng saponins on cytokines in liver fibrosis rats].

作者信息

Jiang Hui, Wang Yong-Zhong, Liu Xiao-Chuang, Xue Xue

机构信息

First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China.

出版信息

Zhong Yao Cai. 2013 Jul;36(7):1123-7.

PMID:24417151

Abstract

OBJECTIVE

To observe the effect of Panax notoginseng saponins (PNS) on cytokines production in liver fibrosis rats and its mechanism.

METHODS

SD rats were randomly divided into 6 groups, including normal group, liver fibrosis model group, PNS (50, 100, 200 mg/kg) treated groups and Col group. Rat liver fibrosis was induced by CCl4 twice a week for 18 weeks. PNS were administered daily from the 9th week for 10 weeks. The IL-1, IL-6, IL-10, TNF-alpha level in serum were determined by radioimmunity. The degree of liver fibrosis was observed by HE and immuninochemistry. The expression of TGF-beta, TNF-alpha mRNA were detected by RT-PCR technology.

RESULTS

Compared with the fibrotic model group, PNS (100, 200 mg/kg) could not only significantly reduce histopathological change, but also effectively decrease the level of IL-1, IL-6, NF-kappaB, TNF-alpha ,TGF-beta, and increase the level of IL-10.

CONCLUSION

PNS has protective effect on liver fibrosis and it maybe related to regulating cytokine network.

摘要

目的

观察三七总皂苷（PNS）对肝纤维化大鼠细胞因子产生的影响及其机制。

方法

将SD大鼠随机分为6组，包括正常组、肝纤维化模型组、PNS（50、100、200mg/kg）治疗组和秋水仙碱组。每周两次皮下注射CCl4诱导大鼠肝纤维化，共18周。从第9周开始每天给予PNS，共10周。采用放射免疫法测定血清中IL-1、IL-6、IL-10、TNF-α水平。通过HE染色和免疫组化观察肝纤维化程度。采用RT-PCR技术检测TGF-β、TNF-αmRNA的表达。