Peng Xiao-dong, Dai Li-li, Huang Chang-quan, He Chun-mei, Yang Bing, Chen Li-juan
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
Biochem Biophys Res Commun. 2009 Oct 9;388(1):31-4. doi: 10.1016/j.bbrc.2009.07.099. Epub 2009 Jul 24.
The aim of this study was to investigate the relationship between anti-fibrotic effect of Panax notoginseng saponins (PNS) and serum cytokines in rat hepatic fibrosis. Hepatic fibrosis induced by carbon tetrachloride (CCl(4)) was studied in animal models using SD rats. Liver index, serum alanine amino transferase (ALT), aspartate amino transferase (AST), transforming growth factor-beta1 (TGF-beta1), tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-10 (IL-10) were measured, respectively. Liver index and the degree of liver fibrosis were also determined. Our results showed that the levels of ALT, AST and liver index in PNS-treated group were markedly lower than those in model group. PNS therapy also significantly attenuated the degree of hepatic fibrosis, collagen area and collagen area percent in liver tissue. Furthermore, the levels of serum TGF-beta1, TNF-alpha and IL-6 were strikingly reduced in PNS-treated group compared with model group while the production of IL-10 was up-regulated. These findings demonstrate that PNS has certain therapeutic effects on hepatic fibrosis probably by immunoregulating the imbalance between pro-fibrotic and anti-fibrotic cytokines.
本研究旨在探讨三七总皂苷（PNS）抗大鼠肝纤维化作用与血清细胞因子之间的关系。采用SD大鼠动物模型研究四氯化碳（CCl₄）诱导的肝纤维化。分别测定肝指数、血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、转化生长因子-β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）。同时测定肝指数和肝纤维化程度。结果显示，PNS治疗组的ALT、AST水平及肝指数均明显低于模型组。PNS治疗还显著减轻了肝纤维化程度、肝组织胶原面积及胶原面积百分比。此外，与模型组相比，PNS治疗组血清TGF-β1、TNF-α和IL-6水平显著降低，而IL-10的产生上调。这些结果表明，PNS可能通过免疫调节促纤维化和抗纤维化细胞因子之间的失衡对肝纤维化具有一定的治疗作用。
