Granado Vera L V, Gutiérrez-Capitán Manuel, Fernández-Sánchez César, Gomes M Teresa S R, Rudnitskaya Alisa, Jimenez-Jorquera Cecilia
CESAM and Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Instituto de Microelectrónica de Barcelona (IMB-CNM), CSIC, 08193 Bellaterra, Barcelona, Spain.
Anal Chim Acta. 2014 Jan 27;809:141-7. doi: 10.1016/j.aca.2013.11.038. Epub 2013 Nov 23.
This work reports on the development of a new voltammetric sensor for diphenylamine based on the use of a miniaturized gold electrode modified with a molecularly imprinted polymer recognition element. Molecularly imprinted particles were synthesized ex situ and further entrapped into a poly(3,4-ethylenedioxythiophene) polymer membrane, which was electropolymerized on the surface of the gold electrode. The thickness of the polymer layer was optimized in order to get an adequate diffusion of the target analyte and in turn to achieve an adequate charge transfer at the electrode surface. The resulting modified electrodes showed a selective response to diphenylamine and a high sensitivity compared with the bare gold electrode and the electrode modified with poly(3,4-ethylenedioxythiophene) and non-imprinted polymer particles. The sensor showed a linear range from 4.95 to 115 μM diphenylamine, a limit of detection of 3.9 μM and a good selectivity in the presence of other structurally related molecules. This sensor was successfully applied to the quantification of diphenylamine in spiked apple juice samples.
本研究报告了一种基于使用分子印迹聚合物识别元件修饰的微型金电极开发的新型二苯胺伏安传感器。分子印迹颗粒在非原位合成，然后进一步包埋在聚（3,4 - 亚乙基二氧噻吩）聚合物膜中，该膜在金电极表面进行电聚合。优化聚合物层的厚度，以便目标分析物有足够的扩散，进而在电极表面实现足够的电荷转移。与裸金电极、聚（3,4 - 亚乙基二氧噻吩）修饰电极和非印迹聚合物颗粒修饰电极相比，所得修饰电极对二苯胺表现出选择性响应和高灵敏度。该传感器对二苯胺的线性范围为4.95至115μM，检测限为3.9μM，在存在其他结构相关分子时具有良好的选择性。该传感器成功应用于加标苹果汁样品中二苯胺的定量分析。
