辐射诱导洋葱蝇 Hylemya antiqua（Meigen）半不育用于遗传控制：II. 新染色体重排的诱导、分离和细胞遗传学分析。
Radiation induced semi-sterility for genetic control purposes in the onion fly Hylemya antiqua (Meigen) : II. Induction, isolation and cytogenetic analysis of new chromosomal rearrangements.
机构信息
Department of Genetics, Agricultural University, Wageningen.
出版信息
Theor Appl Genet. 1974 Jan;45(8):349-54. doi: 10.1007/BF00277675.
The study of the radiobiological and cytogenetic aspects of induced semi-sterility for application in the genetic control of the onion fly Hylemya antiqua (Meigen) has been continued. Doses of 1.5 krad of X-rays or 0.25 krad of fast neutrons were applied to males and 1.0 krad of X-rays or 0.25 krad of fast neutrons to seven day-old females. On the basis of semi-sterility (between 60% and 30% egg hatch) in backcrosses to normal flies, eleven strains were suspected of carrying a chromosomal rearrangement. Seven had a reciprocal translocation and two from a 1.5 krad X-ray treatment showed complex rearrangements. In two strains no rearrangements were found. Two homozygous translocations are described. Combining data of earlier experiments with the new results we concluded that the irradiation of males with low doses, 0.5 krad of X-rays or 0.25 krad of fast neutrons, is suitable for the induction of chromosomal rearrangements. Strains carrying rearrangements from such low dose treatments will be further used for genetic control experiments on the onion fly.
继续研究诱导半不育的放射生物学和细胞遗传学方面，以便将其应用于洋葱蝇 Hylemya antiqua（Meigen）的遗传控制。对雄性应用 1.5 拉德 X 射线或 0.25 拉德快中子，对 7 天大的雌性应用 1.0 拉德 X 射线或 0.25 拉德快中子。根据回交至正常蝇的半不育性（卵孵化率在 60%到 30%之间），有 11 个品系被怀疑携带染色体重排。其中 7 个是相互易位，2 个来自 1.5 拉德 X 射线处理的品系显示出复杂的重排。在两个品系中没有发现重排。描述了两个纯合易位。将早期实验的数据与新结果结合起来，我们得出结论，用低剂量（0.5 拉德 X 射线或 0.25 拉德快中子）照射雄性适合诱导染色体重排。将从这种低剂量处理中携带重排的品系进一步用于洋葱蝇的遗传控制实验。
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