Robinson A S, van Heemert K
Heredity (Edinb). 1981 Feb;46(1):41-8. doi: 10.1038/hdy.1981.4.
In the onion fly, Delia antiqua, a fertile, Y-linked translocation involving chromosomes Y and 2 was irradiated with fast neutrons to induce new complexes involving the Y-chromosome. This chromosome is male determining in the onion fly. Such complexes can be used for the development of genetic sexing systems and also for the introduction of sterility into field populations following release. Irradiation reduced egg fertility by 54 per cent and significantly reduced larval survival but it had no effect on the F1 sex ratio. By measuring the fertility of 807 F1 males following outcrossing, 112 semi-sterile progenies were isolated of which 11 were lost, 29 showed no inheritance of the semi-sterility, 59 were new autosomal translocations and 13 were new complex Y-linked translocations. This classification was accomplished by checking the fertilities of outcrossed F2 males and females. Following cytological observation it was revealed that one of these new complexes involved four chromosome pairs, the remainder involved three. There appeared to be no correlation between the fertility of the translocation and the complexity of the rearrangement. The utilization of these rearrangements in the development of the genetic sexing technique for the onion fly is discussed, together with an assessment of their use for fertility reduction in natural populations.
在葱蝇（Delia antiqua）中，一条涉及Y染色体和2号染色体的可育Y连锁易位染色体被快中子辐照，以诱导涉及Y染色体的新复合体。这条染色体在葱蝇中决定雄性性别。这样的复合体可用于遗传性别鉴定系统的开发，也可用于在释放后使田间种群不育。辐照使卵的育性降低了54%，并显著降低了幼虫的存活率，但对F1代的性别比例没有影响。通过测量807只F1代雄性个体杂交后的育性，分离出112个半不育后代，其中11个丢失，29个未表现出半不育的遗传，59个是新的常染色体易位，13个是新的复合Y连锁易位。这种分类是通过检查杂交F2代雄性和雌性个体的育性来完成的。经过细胞学观察发现，这些新复合体中有一个涉及四对染色体，其余的涉及三对染色体。易位的育性与重排的复杂性之间似乎没有相关性。本文讨论了这些重排在葱蝇遗传性别鉴定技术开发中的应用，以及对它们在降低自然种群育性方面的用途评估。
