水稻Rac/Rop GTP酶OsRac1的纯化、结晶及初步X射线晶体学分析
Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1.
作者信息
Kosami Ken-ichi, Ohki Izuru, Hayashi Kokoro, Tabata Ryo, Usugi Sayaka, Kawasaki Tsutomu, Fujiwara Toshimichi, Nakagawa Atsushi, Shimamoto Ko, Kojima Chojiro
机构信息
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Laboratory of Biophysics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
出版信息
Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):113-5. doi: 10.1107/S2053230X13033645. Epub 2013 Dec 24.
Small GTPases regulate a large variety of key cellular processes. Plant small Rac/Rop GTPases have recently received broad attention as it is becoming clear that these enzymes regulate various plant cellular processes. OsRac1, a rice Rac/Rop protein, is a key regulator of reactive oxygen species (ROS) production and induces immune responses. Although four structures of plant small GTPases have been reported, all of these were of the inactive form. Here, OsRac1 was purified and co-crystallized with the GTP analogue 5'-guanylyl imidodiphosphate (GMPPNP). The crystal belonged to space group P2(1)2(1)2(1) and a complete data set was collected to 1.9 Å resolution.
小GTP酶调节多种关键的细胞过程。植物小Rac/Rop GTP酶最近受到了广泛关注，因为越来越清楚的是，这些酶调节各种植物细胞过程。水稻Rac/Rop蛋白OsRac1是活性氧（ROS）产生的关键调节因子，并诱导免疫反应。尽管已经报道了植物小GTP酶的四种结构，但所有这些都是非活性形式。在这里，OsRac1被纯化并与GTP类似物5'-鸟苷酰亚胺二磷酸（GMPPNP）共结晶。晶体属于空间群P2(1)2(1)2(1)，并收集了完整的数据集，分辨率达到1.9 Å。
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