载体介导的 L-亮氨酸向分离的豌豆（Pisum sativum L.）叶绿体中的运输证据。

Evidence for carrier-mediated transport of L-leucine into isolated pea (Pisum sativum L.) chloroplasts.

机构信息

Department of Physiology and Environmental Studies, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, UK.

出版信息

Planta. 1977 Jan;136(2):147-51. doi: 10.1007/BF00396190.

DOI:10.1007/BF00396190

PMID:24420319

Abstract

The uptake of leucine into isolated, intact, pea chloroplasts was investigated using the silicone oil centrifugation technique. The internal: external ratio of leucine exceeded unity at low external leucine concentrations. Uptake of leucine at different external concentrations showed passive diffusion and carrier-mediated transport components. Competition for uptake was shown between leucine and isoleucine but not between leucine and glycine. Rates of diffusion of leucine were found to be low compared with glycine, however, fast carrier-mediated transport of leucine assumed more importance at physiological concentrations.

摘要

采用硅油离心技术研究了亮氨酸在分离完整豌豆叶绿体中的摄取情况。在低外部亮氨酸浓度下，亮氨酸的内部：外部比例超过了 1。在不同的外部浓度下，亮氨酸的摄取显示出被动扩散和载体介导的运输成分。亮氨酸和异亮氨酸之间存在竞争摄取，但亮氨酸和甘氨酸之间不存在竞争摄取。与甘氨酸相比，亮氨酸的扩散速度较慢，然而，在生理浓度下，亮氨酸的快速载体介导运输显得更为重要。