Osman Kamelia M, Ali Mona M, Radwan Moustafa I, Kim Hyoung Kyu, Han Jin
Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
J Proteomics. 2009 Jul 21;72(5):815-21. doi: 10.1016/j.jprot.2009.05.002. Epub 2009 May 25.
Comparative proteomics analysis of the cytosolic proteins of Salmonella Gallinarum (SG) and Salmonella Enteritidis (SE) isolated from poultry was performed. The constantly detected spots of serovar SG with concomitant absence in SE serovar as well as those markedly over expressed in serovar SE were selected for MALDI-TOF-MS identification. The NCBI-matched proteins that show overregulation were then further confirmed on the mRNA level by quantitative real time PCR. Identified proteins were representing diverse functional activities including energy production, metabolism, and nucleic acid synthesis. Interestingly, some recognized proteins have some relevance to bacterial virulence e.g. Salmonella pathogenicity island 1 effector protein, T-cell inhibitor protein, response regulator protein, paratose synthetase protein (RfbS) and heat shock protein 90. The study revealed the presence of some proteins of unknown function, which raise the speculation for their importance in either host adaptation or pathogenicity among SG serovars.
对从家禽中分离出的鸡沙门氏菌（SG）和肠炎沙门氏菌（SE）的胞质蛋白进行了比较蛋白质组学分析。选择在血清型SG中持续检测到而在血清型SE中不存在的斑点，以及在血清型SE中明显过度表达的斑点进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定。然后通过定量实时PCR在mRNA水平上进一步确认显示上调的NCBI匹配蛋白。鉴定出的蛋白质代表了多种功能活动，包括能量产生、代谢和核酸合成。有趣的是，一些识别出的蛋白质与细菌毒力有一定关联，例如沙门氏菌致病岛1效应蛋白、T细胞抑制蛋白、反应调节蛋白、副糖合成酶蛋白（RfbS）和热休克蛋白90。该研究揭示了一些功能未知的蛋白质的存在，这引发了对它们在SG血清型的宿主适应性或致病性中重要性的推测。
