Department of Sport Medicine (J.K., L.R., Z.K., E.K., J.P., M.T., L.M., V.S., M.S.) and Franco-Czech Laboratory for Clinical Research on Obesity (J.K., L.R., Z.K., E.K., M.T., L.M., V.S., D.L., M.S.), Third Faculty of Medicine, Charles University, 100 00 Prague 100 00 Czech Republic; INSERM, 31059 Toulouse, France; INSERM, Unité Mixte de Recherche 1048 (D.L.), Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France; University of Toulouse, Unité Mixte de Recherche 1048 (D.L.), Paul Sabatier University, 31432 Toulouse, France; and Department of Clinical Biochemistry (D.L.), Toulouse University Hospitals, 31000 Toulouse, France.
J Clin Endocrinol Metab. 2014 Mar;99(3):E528-35. doi: 10.1210/jc.2013-3348. Epub 2014 Jan 13.
Soluble CD163 (sCD163) was suggested as a biomarker of insulin sensitivity and CD163 mRNA expression representing macrophage content in adipose tissue (AT).
The aim of this study was to investigate, in cross-sectional and prospective design, the relationship between sCD163 circulating levels and CD163 mRNA expression in adipose tissue and insulin sensitivity assessed by euglycemic-hyperinsulinemic clamp.
DESIGN, SETTING, PARTICIPANTS, AND INTERVENTIONS: Two cohorts of subjects were examined in the study. Cohort 1 included 42 women with a wide range of body mass index (17-48 kg/m(2)); cohort 2 included 27 obese women who followed a dietary intervention consisting of 1 month of a very low-calorie diet and 5 months of a weight-stabilization period.
Serum levels of CD163 and mRNA expression of CD163 and CD68 in sc and visceral (visc) AT were determined, and insulin sensitivity [expressed as glucose disposal rate (GDR)] was measured in cohort 1. In cohort 2, serum levels of CD163, mRNA expressions of CD163, CD68, and CD163-shedding factors [TNF-α-converting enzyme (TACE) and tissue inhibitor of metalloproteinase (TIMP3)] in sc AT were examined and GDR was measured before and during dietary intervention.
In cohort 1, circulating sCD163 correlated with CD163 mRNA levels in both sc and visc AT. sCD163 and CD163 mRNA expression in both fat depots correlated with GDR. In cohort 2, the diet-induced changes of sCD163 levels did not correlate with those of CD163, CD68, TACE, and TIMP3 mRNA levels. Although the pattern of the diet-induced change of sCD163 paralleled that of GDR, there was no correlation between the changes of these two variables.
sCD163 correlates with CD163 mRNA expression in sc and visc AT and with whole-body insulin sensitivity in the steady-state condition. These associations are not observed with respect to the diet-induced changes during a weight-reducing hypocaloric diet.
可溶性 CD163（sCD163）被认为是胰岛素敏感性的生物标志物，CD163mRNA 的表达代表了脂肪组织（AT）中的巨噬细胞含量。
本研究旨在通过正葡萄糖高胰岛素钳夹试验评估胰岛素敏感性，以横断面和前瞻性设计研究循环 sCD163 水平与脂肪组织中 CD163mRNA 表达之间的关系。
设计、地点、参与者和干预措施：本研究检查了两组受试者。队列 1 包括 42 名女性，其体重指数（BMI）范围很广（17-48kg/m²）；队列 2 包括 27 名肥胖女性，她们遵循了为期 1 个月的极低卡路里饮食和 5 个月的体重稳定期的饮食干预。
测定血清 CD163 水平和 sc 及内脏（visc）AT 中 CD163 和 CD68mRNA 的表达，并在队列 1 中测量胰岛素敏感性（以葡萄糖处置率（GDR）表示）。在队列 2 中，在饮食干预之前和期间，测定 scAT 中血清 CD163、CD163、CD68 和 CD163 脱落因子（TNF-α转化酶（TACE）和金属蛋白酶组织抑制剂（TIMP3））的 mRNA 表达，并测量 GDR。
在队列 1 中，循环 sCD163 与 sc 和 viscAT 中 CD163mRNA 水平相关。两个脂肪组织中 sCD163 和 CD163mRNA 的表达与 GDR 相关。在队列 2 中，sCD163 水平的饮食诱导变化与 CD163、CD68、TACE 和 TIMP3mRNA 水平的变化无关。尽管 sCD163 的饮食诱导变化模式与 GDR 相似，但这两个变量之间没有相关性。
sCD163 与 sc 和 viscAT 中的 CD163mRNA 表达以及稳态条件下的全身胰岛素敏感性相关。在减肥低热量饮食期间，这些关联与饮食诱导的变化无关。
