Mihaiu Liora, Lapusan Alexandra, Tanasuica Rodica, Sobolu Rodica, Mihaiu Romolica, Oniga Ovidiu, Mihaiu Marian
Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
J Infect Dev Ctries. 2014 Jan 15;8(1):50-8. doi: 10.3855/jidc.3715.
The increasing antimicrobial resistance of Salmonella isolates is of major public health concern, but information regarding these aspects is still lacking in Romania. This study focused on a detailed and accurate investigation concerning prevalence, serotypes, and antimicrobial resistance patterns of Salmonella strains, isolated from pork and chicken meat, collected from all regions of Romania in 2011.
The research was conducted on 650 samples of chicken and pork meat collected from production units and retail markets located in various regions of Romania. A total of 149 Salmonella isolates were recovered (22.92%), serotyped, confirmed by PCR, and further tested for antimicrobial susceptibility.
Thirteen Salmonella serovars were identified; predominant serovars included Infantis, Typhimurium, Derby and Colindale. Multiple resistance was found in 83.22% (n = 124) of the isolates. The isolates were frequently resistant to tetracycline (80.53%), streptomycin (81.21%), sulfamethoxazole (87.25%), nalidixic acid (65.10%), and ciprofloxacin (42.95%). Additionally, a markedly lower resistance rate was observed for ampicillin (20.81%), chloramphenicol (16.78%), and ceftazidime (11.41%). Among 137 resistant Salmonella isolates, 35 different resistance patterns were found.
A high prevalence of Salmonella spp. and a relatively high resistance rate to multiple antimicrobials was found. This data indicates that chicken and pork meat could constitute a source of human exposure to multidrug-resistant Salmonella and therefore could be considered a potential vehicle of resistant Salmonella foodborne diseases. Further actions are needed to succesfully implement a national surveillance program for better monitoring of these resistant pathogens.
沙门氏菌分离株日益增加的抗菌药物耐药性是主要的公共卫生问题，但罗马尼亚仍缺乏有关这些方面的信息。本研究着重对2011年从罗马尼亚所有地区采集的猪肉和鸡肉中分离出的沙门氏菌菌株的流行率、血清型和抗菌药物耐药模式进行详细而准确的调查。
对从罗马尼亚不同地区的生产单位和零售市场采集的650份鸡肉和猪肉样本进行了研究。共分离出149株沙门氏菌（22.92%），进行血清分型、PCR确认，并进一步检测其抗菌药物敏感性。
鉴定出13种沙门氏菌血清型；主要血清型包括婴儿型、鼠伤寒型、德比型和柯林代尔型。83.22%（n = 124）的分离株发现多重耐药。这些分离株经常对四环素（80.53%）、链霉素（81.21%）、磺胺甲恶唑（87.25%）、萘啶酸（65.10%）和环丙沙星（42.95%）耐药。此外，氨苄西林（20.81%）、氯霉素（16.78%）和头孢他啶（11.41%）的耐药率明显较低。在137株耐药沙门氏菌分离株中，发现了35种不同的耐药模式。
发现沙门氏菌属的高流行率和对多种抗菌药物的相对较高耐药率。这些数据表明，鸡肉和猪肉可能构成人类接触多重耐药沙门氏菌的来源，因此可被视为耐药沙门氏菌食源性疾病的潜在传播媒介。需要采取进一步行动，以成功实施国家监测计划，更好地监测这些耐药病原体。
